環保專案成果報告查詢系統

專案計畫編號	EPA-99-E3S4-02-02
經費年度	099
計畫經費	1,436 千元
專案開始日期	2010/01/01
專案結束日期	2010/12/31
專案主持人	陳家揚
主辦單位	環檢所
承辦人	趙春美
執行單位	國立台灣大學
專案分類	環境監測及檢測
中文關鍵字	個人保健品，液相層析串聯式質譜儀，生活污水，表面水，固相萃取
英文關鍵字	Personal care products, LC/MS/MS, Sewage, Surface water, Solid-phase extraction
協同主持人	龍世俊
共同主持人
計畫聯絡人	陳家揚
計畫聯絡電話	02-33668101#
計畫聯絡信箱	dbms@ntu.edu.tw

中文摘要	個人保健品為日常生活中民眾基於健康照護、提神、美容、殺菌等目的而使用之個人或居家用品。這些藥品或成分於使用後或是因任意棄置，可經由多重管道進入環境中；雖然個別待測物濃度也許不高，但對其水中生物或人類健康的影響仍然值得持續關注。本計畫針對鎮痛解熱劑、荷爾蒙及作用類似物、清潔用品成分、防曬劑、美容用品、驅蟲劑、興奮劑、保健食品與草藥類等八大類共29待測物，包括diclofenac、indomethacine、ibuprofen、acetaminophen、ketoprofen、naproxen、acetylsalicylic acid、fenoprofen、aminopyrine、phenazone、meclofenamic acid、17α-ethynyl estradiol、estrone、17β-estradiol、estriol、finasteride、triclosan、4-chloro-3,5-xylenol、nonylphenol (NP)、oxybenzone、methylbenzylidene camphor、benzophenone、methyl paraben、ethyl paraben、propyl paraben、butyl paraben、DEET、caffeine、huperzine A，完成水樣前處理技術開發以及液相層析/質譜/質譜儀分析方法建立。 500 mL之水樣以PolarPlus C18 （50 mm）固相萃取圓盤萃取，以甲醇及二氯甲烷沖提，沖提液經濃縮至0.5 mL後加入0.3 mL試劑水再濃縮至0.5 mL，以極致液相層儀(ultra-performance liquid chromatography, UPLC）搭配串聯式質譜儀和電灑游離（ESI）分析。正電荷離子使用Kinetex PFP（50 × 2.1 mm, 2.6 μm）管柱分析，負電荷則為Ascentis Express C18（50 × 2.1 mm, 2.7 μm）進行層析。採樣地點為南部主要河川八掌溪（輕度污染）、鹽水溪（嚴重污染）及高屏溪（稍受污染至嚴重污染）之重要支流和其本流以及北部三座污水處理場之進流水、初級處理後污水、二級處理後污水及放流水共計31個水體樣本。水體檢測結果顯示，acetaminphen、finasteride、oxybenzone、benzophenone、nonylphenol、DEET、caffeine、methyl paraben、butyl paraben於表面水體檢出率高（>77%）。濃度方面，以鹽水溪橋之acetaminphen (338 ng/L)、oxybenzone (242 ng/L)；新灣橋之benzophenone (753 ng/L)；嘉南大橋之NP (756 ng/L)等待測物濃度較高，顯示多種個人保健品已存在於南部的河川流域。在三個污水處理廠的進流水樣本中，以acetaminophen與caffeine濃度最高；acetaminophen分別為32,449 ng/L、22,131 ng/L及26,250 ng/L，caffeine分別為13,084 ng/L、14,362 ng/L及25,207 ng/L。
中文關鍵字	個人保健品，液相層析串聯式質譜儀，生活污水，表面水，固相萃取

中文摘要

個人保健品為日常生活中民眾基於健康照護、提神、美容、殺菌等目的而使用之個人或居家用品。這些藥品或成分於使用後或是因任意棄置，可經由多重管道進入環境中；雖然個別待測物濃度也許不高，但對其水中生物或人類健康的影響仍然值得持續關注。本計畫針對鎮痛解熱劑、荷爾蒙及作用類似物、清潔用品成分、防曬劑、美容用品、驅蟲劑、興奮劑、保健食品與草藥類等八大類共29待測物，包括diclofenac、indomethacine、ibuprofen、acetaminophen、ketoprofen、naproxen、acetylsalicylic acid、fenoprofen、aminopyrine、phenazone、meclofenamic acid、17α-ethynyl estradiol、estrone、17β-estradiol、estriol、finasteride、triclosan、4-chloro-3,5-xylenol、nonylphenol (NP)、oxybenzone、methylbenzylidene camphor、benzophenone、methyl paraben、ethyl paraben、propyl paraben、butyl paraben、DEET、caffeine、huperzine A，完成水樣前處理技術開發以及液相層析/質譜/質譜儀分析方法建立。 500 mL之水樣以PolarPlus C18 （50 mm）固相萃取圓盤萃取，以甲醇及二氯甲烷沖提，沖提液經濃縮至0.5 mL後加入0.3 mL試劑水再濃縮至0.5 mL，以極致液相層儀(ultra-performance liquid chromatography, UPLC）搭配串聯式質譜儀和電灑游離（ESI）分析。正電荷離子使用Kinetex PFP（50 × 2.1 mm, 2.6 μm）管柱分析，負電荷則為Ascentis Express C18（50 × 2.1 mm, 2.7 μm）進行層析。採樣地點為南部主要河川八掌溪（輕度污染）、鹽水溪（嚴重污染）及高屏溪（稍受污染至嚴重污染）之重要支流和其本流以及北部三座污水處理場之進流水、初級處理後污水、二級處理後污水及放流水共計31個水體樣本。水體檢測結果顯示，acetaminphen、finasteride、oxybenzone、benzophenone、nonylphenol、DEET、caffeine、methyl paraben、butyl paraben於表面水體檢出率高（>77%）。濃度方面，以鹽水溪橋之acetaminphen (338 ng/L)、oxybenzone (242 ng/L)；新灣橋之benzophenone (753 ng/L)；嘉南大橋之NP (756 ng/L)等待測物濃度較高，顯示多種個人保健品已存在於南部的河川流域。在三個污水處理廠的進流水樣本中，以acetaminophen與caffeine濃度最高；acetaminophen分別為32,449 ng/L、22,131 ng/L及26,250 ng/L，caffeine分別為13,084 ng/L、14,362 ng/L及25,207 ng/L。

中文關鍵字

個人保健品，液相層析串聯式質譜儀，生活污水，表面水，固相萃取

類型	檔名	檔案大小	說明	最新版公開日期
期末報告	計畫成果摘要（詳細版）.pdf	0MB		2011/2/1 上午 12:00:00

摘要	Personal care products (PCPs) are used for daily health care, refreshment, cosmetology, and disinfection etc. The compounds or products may be disposed improperly then enter the environment. Although the concentrations of PCPs were usually at trace amount in waters, it is still a concern on their potential adverse effects on aquatic creatures and human health. This project targeted 29 critical chemicals at eight categories, including analgesics, hormones, ingredients in detergent/disinfectant, suntan lotions, preservatives in cosmetics, repellent, stimulant, and health food with herbal medicine, which included diclofenac、indomethacine、ibuprofen、acetaminophen、ketoprofen、naproxen、acetylsalicylic acid、fenoprofen、aminopyrine、phenazone、meclofenamic acid、17α-ethynyl estradiol、estrone、17β-estradiol、estriol、finasteride、triclosan、4-chloro-3,5-xylenol、nonylphenol (NP)、oxybenzone、methylbenzylidene camphor、benzophenone、methyl paraben、ethyl paraben、propyl paraben、butyl paraben、DEET、caffeine、huperzine A. A method to extract them from water and followed by the determination using ultra-performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS) with electrospray ionization (ESI) has been developed and validated. Water samples were extracted with 50-mm PolarPlus C18 speedisk using automatied solid-phase extraction system, and eluted with methanol and dichloromethane. The eluent was concentrated to 0.5 mL and added 0.3 mL milli-Q water and then concentrated to 0.5 mL. Analytes forming positive ions were separated on a Kinetex PFP column (50 × 2.1 mm, 2.6 μm), and chemicals forming negative ions were done on a Ascentis Express C18 column (50 × 2.1 mm, 2.7 μm). Water samples were collected from 22 representative points of three main streams in Southern Taiwan. In addition, wastewater samples from influents, after primary treatment, after secondary treatment, and effluents were grabbed from three wastewater treatment plants in Northern Taiwan. The compounds of acetaminphen、finasteride、oxybenzone、benzophenone、nonylphenol、DEET、caffeine、methyl paraben and butyl paraben were detected in more than 77% of surface water samples. The highest concentrations were found to be acetaminphen 338 ng/L, oxybenzone 242 ng/L, benzophenone 753 ng/L and NP 756 ng/L. The results indicated that PCPs would have been widely distributed in rivers of the southern Taiwan. The highest concentrations of compounds found in influent of sewage treatment plants were acetaminophen and caffeine. Acetaminophen concentration was 32,449 ng/L, 22,131 ng/L and 26,250 ng/L, caffeine concentration was 13,084 ng/L and 14,362 ng/L and 25,207 ng/L.
英文關鍵字	Personal care products, LC/MS/MS, Sewage, Surface water, Solid-phase extraction

摘要

Personal care products (PCPs) are used for daily health care, refreshment, cosmetology, and disinfection etc. The compounds or products may be disposed improperly then enter the environment. Although the concentrations of PCPs were usually at trace amount in waters, it is still a concern on their potential adverse effects on aquatic creatures and human health. This project targeted 29 critical chemicals at eight categories, including analgesics, hormones, ingredients in detergent/disinfectant, suntan lotions, preservatives in cosmetics, repellent, stimulant, and health food with herbal medicine, which included diclofenac、indomethacine、ibuprofen、acetaminophen、ketoprofen、naproxen、acetylsalicylic acid、fenoprofen、aminopyrine、phenazone、meclofenamic acid、17α-ethynyl estradiol、estrone、17β-estradiol、estriol、finasteride、triclosan、4-chloro-3,5-xylenol、nonylphenol (NP)、oxybenzone、methylbenzylidene camphor、benzophenone、methyl paraben、ethyl paraben、propyl paraben、butyl paraben、DEET、caffeine、huperzine A. A method to extract them from water and followed by the determination using ultra-performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS) with electrospray ionization (ESI) has been developed and validated. Water samples were extracted with 50-mm PolarPlus C18 speedisk using automatied solid-phase extraction system, and eluted with methanol and dichloromethane. The eluent was concentrated to 0.5 mL and added 0.3 mL milli-Q water and then concentrated to 0.5 mL. Analytes forming positive ions were separated on a Kinetex PFP column (50 × 2.1 mm, 2.6 μm), and chemicals forming negative ions were done on a Ascentis Express C18 column (50 × 2.1 mm, 2.7 μm). Water samples were collected from 22 representative points of three main streams in Southern Taiwan. In addition, wastewater samples from influents, after primary treatment, after secondary treatment, and effluents were grabbed from three wastewater treatment plants in Northern Taiwan. The compounds of acetaminphen、finasteride、oxybenzone、benzophenone、nonylphenol、DEET、caffeine、methyl paraben and butyl paraben were detected in more than 77% of surface water samples. The highest concentrations were found to be acetaminphen 338 ng/L, oxybenzone 242 ng/L, benzophenone 753 ng/L and NP 756 ng/L. The results indicated that PCPs would have been widely distributed in rivers of the southern Taiwan. The highest concentrations of compounds found in influent of sewage treatment plants were acetaminophen and caffeine. Acetaminophen concentration was 32,449 ng/L, 22,131 ng/L and 26,250 ng/L, caffeine concentration was 13,084 ng/L and 14,362 ng/L and 25,207 ng/L.

英文關鍵字

Personal care products, LC/MS/MS, Sewage, Surface water, Solid-phase extraction