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專案計畫編號	EPA-103-1603-02-02
經費年度	103
計畫經費	1,500 千元
專案開始日期	2014/03/19
專案結束日期	2014/12/31
專案主持人	施養信
主辦單位	環檢所
承辦人	黃豊文
執行單位	國立臺灣大學農業化學系
專案分類	環境監測及檢測
中文關鍵字	奈米微粒，動態光散射技術，離心，流力層析，電子顯微鏡
英文關鍵字	nanoparticles, dynamic light scattering, centrifugation, hydrodynamic chromatography, electron microscopy
協同主持人
共同主持人
計畫聯絡人	1. 施養信 2. 張穎捷
計畫聯絡電話	02-33669442#
計畫聯絡信箱	1. yhs@ntu.edu.tw 2. waterman79926@hotmail.com

中文摘要	本研究之主要目的在於建立水環境中奈米微粒量測技術，即如何應用測量技術量測水體中奈米微粒大小尺寸與濃度。購置四種市售商用奈米銀懸浮液，以動態光散射儀(dynamic light scattering, DLS)量測平均粒徑，奈米銀A、B與C之平均粒徑分別為79.86、80.6及122 nm，而奈米銀D則為1663 nm，其顆粒大小較為不均勻。採集三處廢水水樣及三處環境水樣，其所含顆粒之粒徑大小分布不均勻，含不同離子濃度，pH介於6.9-8.5，導電度在375-11200 µS/cm。奈米銀於不同水質之穩定性，發現奈米銀有不同程度的聚集與溶解，建議環境樣品應置於低溫、不調整pH值且避光的條件下保存，亦應儘速分析。樣品量測前處理建議以離心速度2000 G離心2分鐘以去除溶液中大顆粒物質，回收率為71 %，並且不建議以過濾作為樣品前處理之程序。奈米微粒濃度量測前，以70 %濃硝酸消化再以感應耦合電漿質譜儀(inductively coupled plasma-mass spectrometer, ICP-MS)定量為最合適的處理方式。流力層析(hydrodynamic chromatography, HDC)以pH 10之純水作為流洗液時，分離效果良好。在純水下所建立之標準曲線能計算不同水體中的奈米銀粒徑，HDC測值與DLS之測值大致吻合，同時可輔以電子顯微鏡(electron microscopy)確認之。研究結果初步建立了HDC分離無機奈米微粒與DLS量測奈米粒徑技術之奈米量測方法草案。
中文關鍵字	奈米微粒，動態光散射技術，離心，流力層析，電子顯微鏡

中文摘要

本研究之主要目的在於建立水環境中奈米微粒量測技術，即如何應用測量技術量測水體中奈米微粒大小尺寸與濃度。購置四種市售商用奈米銀懸浮液，以動態光散射儀(dynamic light scattering, DLS)量測平均粒徑，奈米銀A、B與C之平均粒徑分別為79.86、80.6及122 nm，而奈米銀D則為1663 nm，其顆粒大小較為不均勻。採集三處廢水水樣及三處環境水樣，其所含顆粒之粒徑大小分布不均勻，含不同離子濃度，pH介於6.9-8.5，導電度在375-11200 µS/cm。奈米銀於不同水質之穩定性，發現奈米銀有不同程度的聚集與溶解，建議環境樣品應置於低溫、不調整pH值且避光的條件下保存，亦應儘速分析。樣品量測前處理建議以離心速度2000 G離心2分鐘以去除溶液中大顆粒物質，回收率為71 %，並且不建議以過濾作為樣品前處理之程序。奈米微粒濃度量測前，以70 %濃硝酸消化再以感應耦合電漿質譜儀(inductively coupled plasma-mass spectrometer, ICP-MS)定量為最合適的處理方式。流力層析(hydrodynamic chromatography, HDC)以pH 10之純水作為流洗液時，分離效果良好。在純水下所建立之標準曲線能計算不同水體中的奈米銀粒徑，HDC測值與DLS之測值大致吻合，同時可輔以電子顯微鏡(electron microscopy)確認之。研究結果初步建立了HDC分離無機奈米微粒與DLS量測奈米粒徑技術之奈米量測方法草案。

中文關鍵字

奈米微粒，動態光散射技術，離心，流力層析，電子顯微鏡

類型	檔名	檔案大小	說明	最新版公開日期
期末報告	103 EPA期末報告定稿.pdf	4MB		2015/5/1 上午 12:00:00

摘要	This study aims to establish a measuring technique for the inorganic engineered nanoparticles in the aquatic environment, including how to apply the analytical technologies to determine the size distribution and concentration of nanoparticles in water. The average particle sizes of three commercial silver nanoparticles (AgNPs) suspensions measured by dynamic light scattering (DLS) were 79.86, 80.6 and 122 nm for AgNP-A, B and C, and 1663 nm was for AgNP-D. In three wastewater samples and three environmental samples, the particle sizes were multimodal distribution. They have different ion contents, the pH values were around 6.9-8.5 and the conductivities were between 375-11200 μS/cm. The stability of AgNPs was evaluated by adding AgNPs into these samples. Different levels of aggregation and dissolution were observed. Samples should be stored under a lower temperature such as 4℃ without pH adjustment, in the dark, and analyzed as soon as possible. For the pretreatment process, the separation of different particle sizes was suggested by the centrifugation with centrifugal speed of 4000 G for 2 minutes, which has 71 % recovery. The filtration is not suggested as a pretreat process. To quantify the concentration of nanoparticles, the best process is to use 70 % nitric acid to digest samples before inductively coupled plasma-mass spectrometer (ICP-MS) analysis. For the HDC analysis, the correlation between particle size and retention time was determined. pH 10 pure water was chosen as the eluent for the HDC experiment due to its good correlation coefficient. The calibration curves established in pure water can predict the size of AgNPs in different water samples and compare well to the size results from DLS. The size of silver in the recovered eluent could be confirmed by electron microscopy. The results of this study establish a preliminary technique to measure the nanoparticles in aquatic environment and a draft for DLS and HDC analysis.
英文關鍵字	nanoparticles, dynamic light scattering, centrifugation, hydrodynamic chromatography, electron microscopy

摘要

This study aims to establish a measuring technique for the inorganic engineered nanoparticles in the aquatic environment, including how to apply the analytical technologies to determine the size distribution and concentration of nanoparticles in water. The average particle sizes of three commercial silver nanoparticles (AgNPs) suspensions measured by dynamic light scattering (DLS) were 79.86, 80.6 and 122 nm for AgNP-A, B and C, and 1663 nm was for AgNP-D. In three wastewater samples and three environmental samples, the particle sizes were multimodal distribution. They have different ion contents, the pH values were around 6.9-8.5 and the conductivities were between 375-11200 μS/cm. The stability of AgNPs was evaluated by adding AgNPs into these samples. Different levels of aggregation and dissolution were observed. Samples should be stored under a lower temperature such as 4℃ without pH adjustment, in the dark, and analyzed as soon as possible. For the pretreatment process, the separation of different particle sizes was suggested by the centrifugation with centrifugal speed of 4000 G for 2 minutes, which has 71 % recovery. The filtration is not suggested as a pretreat process. To quantify the concentration of nanoparticles, the best process is to use 70 % nitric acid to digest samples before inductively coupled plasma-mass spectrometer (ICP-MS) analysis. For the HDC analysis, the correlation between particle size and retention time was determined. pH 10 pure water was chosen as the eluent for the HDC experiment due to its good correlation coefficient. The calibration curves established in pure water can predict the size of AgNPs in different water samples and compare well to the size results from DLS. The size of silver in the recovered eluent could be confirmed by electron microscopy. The results of this study establish a preliminary technique to measure the nanoparticles in aquatic environment and a draft for DLS and HDC analysis.

英文關鍵字

nanoparticles, dynamic light scattering, centrifugation, hydrodynamic chromatography, electron microscopy