EPA-104-1604-02-02

104

1,900 千元

2015/03/30

2015/12/31

賴宣婷

環檢所

陳怡如

美商傑明工程顧問(股)台灣分公司

土壤及地下水

拉曼光譜、土壤、糙米

Raman microspectrometry, soil, rice

王俊凱

廖珮瑜

02-23252100#20

tiffany.liao@mwhglobal.com

為釐清稻作在不同鉛傳輸途徑下，於不同生長期期間，稻根、稻葉、稻莖及糙米各部位中不同形態鉛的分布情形，遂執行「拉曼光譜應用於環境污染物之傳輸途徑研究計畫」（以下簡稱為本計畫），希冀透過稻作試驗工作，建立土壤及稻作樣品重金屬鉛拉曼光譜分析技術，並掌握植體各部位之拉曼光譜特性差異。 本計畫自104年5月29日起於台灣大學生物資源暨農學院附設人工氣候室進行盆栽試驗。以重金屬鉛背景值濃度與高濃度（1,000 mg/kg以上）之土壤，以及是否噴施硝酸鉛水溶液（500 mg Pb/L）提供葉面鉛源進行試驗，設計有試驗對照組、根部試驗組、葉面試驗組及根葉綜合試驗組共4組試驗組，每試驗組皆種植4個盆栽，供稻作4個生長時期（分糵期、生長中期、生長後期及糊熟期）採樣，總計有16個盆栽。同時已完成稻作4個生長期之土壤、稻根、稻莖、稻葉及稻米採樣與重金屬鉛全量分析，其中土壤分析數量為16組、稻作植體分析數量為48組，共計64組重金屬鉛全量分析。另外，本計畫應用拉曼光譜分析土壤及稻作植體，已完成39組土壤分析、3組植體分析，共計42組拉曼光譜分析。故，本計畫完成總計106組樣品分析工作。另外，本計畫亦完成10篇國內外文獻資料蒐集與回顧。 本計畫嘗試先選取重金屬鉛全量分析濃度最高的根葉綜合試驗組之樣品進行植體拉曼光譜分析，而分析部位以根部、葉部為主。操作參數為785 nm雷射搭配10倍物鏡，雷射強度約為15 mW，結果顯示根部表皮的拉曼光譜可以馬上辨識出有四個在247 cm-1、376 cm-1、1268 cm-1及1575 cm-1的特徵拉曼波峰。值得一提是，247 cm-1及376 cm-1兩個特徵拉曼波峰與國外研究指出Pb-S振動拉曼訊號的可能頻率很接近，並且與使用其它螢光方法所得到鉛主要存在於稻根表面的結論一致(P. N. Williams et al., 2014)。惟仍須更進一步分析才能確定兩個特徵拉曼波峰的來源。另外，在葉片氣孔所量得拉曼光譜並無硝酸鉛的拉曼光譜，目前並無法知道原因，仍須再深入研究。 本計畫針對硝酸鉛粉末，利用給予不同激發功率，建立激發功率與訊號強度之關係，並利用較佳操作條件，分析不同含鉛樣品，包含粉狀藥品（含鉛標準品）、含鉛礦物及模擬污染土壤（鉛混合石英砂）等，以獲得不同樣品型態（粉末、礦物及土壤）及分析區域（平面、不同顏色及明暗處等）之拉曼訊號值及特徵波數，並以此建立鉛標準品拉曼光譜之資料庫，作為未來分析污染土壤中鉛型態之依據。

拉曼光譜、土壤、糙米

EPA-104-1604-02-02.pdf

26MB

2016/2/1 上午 12:00:00

Foliar uptake experiment was conducted in this project of “The Application of Raman Microspectrometry in Environmental Forensics”. The experiment was conducted in Phytotron of National Taiwan University between May 29th and September 17th, 2015. The experiment considered different concentrations of Pb in soil (background value and 1,000 mg/kg) and with or without spraying lead nitrate solution on leaves (500 mg Pb/L). There were a total of 16 experimental pots, including 1control group and 3 experimental groups (root group, leaf group, and root-leaf combination group), each with 4 pots (for 4 crop growing stages). Total Pb concentration was analyzed for soil, root, shoot, leaf, and rice, with a total of 64 samples. Further, the project completed 39 soil analysis and 3 plant tissue analysis of Raman Microspectrometry. To sum up, there were 106 sample analysis in total. In addition, 10 literature reviews were also completed. The root sample from the root-leaf combination group was analyzed by Raman Microspectrometry fitted with a 785 nm excitation source, 10X magnification, and laser power of 15 mW. A total of 4 peaks were detected at 247 cm-1、376 cm-1、1268 cm-1及1575 cm-1. It is noted that 247 cm-1 and 376 cm-1 may be resulted from Pb-S bonding (P. N. Williams et al., 2014). Pb(NO3)2, PbSO4, PbO, cerussite, and Pb-containing sand were also analyzed by Raman Microspectrometry fitted with a 532 nm excitation source, 10X magnification, and various laser power. Contaminated soil was analyzed by Raman Microspectrometry fitted with a 785 nm excitation source, 10X magnification, and laser power of 24.9 mW.

Raman microspectrometry, soil, rice