EPA-104-E3S5-02-03

104

1,600 千元

2015/04/16

2015/12/31

闕斌如

環檢所

廖儀如

國立中興大學

環境監測及檢測

奈米微粒，細胞毒性，細胞電阻檢測，空氣細懸浮微粒

Nanoparticles, cytotoxicity, cell impedance measurement, PM2.5

莊秀美

闕斌如

04-22840896#120

pjchueh@dragon.nchu.edu.tw

全球奈米科技正如火如荼的發展，雖然此尖端科技在各個領域上開拓新的希望、應用價值與新興產品，然而這些具有奈米結構的物質，其本身的化學性質與物理性質，會隨著粒徑大小的不同而有所變化，對人體的毒性危害或影響也可能有所改變，因此對於安全衛生環保亦將可能產生重大的衝擊。本團隊自100年度起，開始建立新穎之奈米微粒細胞毒性篩選平台，持續開發符合OECD推薦之以細胞電阻為主的檢測技術，並利用市售之奈米金為標的，驗證此細胞毒性篩選平台具較穩定、快速及可測試大量奈米微粒樣品等特質。我們也認為此篩選平台不僅可評估奈米微粒對細胞之危害性，也可應用到環境其他危害因子對生物體的影響評估。近年來由於空氣品質而導致的呼吸及心血管系統的疾病日益增多，世界各地已將焦點放在空氣中的懸浮粒子，由於懸浮或細懸浮粒子(PM2.5) 能夠在大氣中停留很長時間，並可隨呼吸進入體內，造成危害影響身體健康。因此在今年度的計畫，配合由環檢所提供之環境監測取得的空氣樣品，建立以細胞電阻檢測分析並了解空氣中細懸浮粒子PM2.5樣品對細胞生物危害程度，並以傳統之細胞增生試驗來佐證結果，另外也利用氧化壓力測試及細胞週期或細胞凋亡分析評估其對細胞的傷害；並利用細胞電阻檢測技術，分析其他環境整治所用的奈米物質如施威特曼石對細胞的生物影響。利用我們建立的平台發現，環境整治所用的奈米施威特曼石在本系統中對細胞的生物影響並不大，未來可能具有在環境整治上的應用價值；目前已就現有的空氣中細懸浮粒子PM2.5樣品產生對細胞生物危害程度的初步結果，但我們希望能在下一年度繼續分析以釐清其重量濃度、氧化壓力產生、發炎因子釋放及對細胞生物影響間的關係。

奈米微粒，細胞毒性，細胞電阻檢測，空氣細懸浮微粒

EPA104-E3S50203.pdf

1MB

2018/12/25 上午 12:00:00

Nanomaterials have received considerable recent attention because of their unique properties and diverse applications in technology and life sciences. However, the nano-structures of these materials possess very different physical and chemical properties compared to their larger size counterparts. Given the increasing use of nanoparticles in a range of products, it has become crucial to develop a fundamental understanding of the biological effects of nanoparticle exposure. For the past years, we have established an in vitro cytotoxicity screening platform measuring cell impedance. Due to the increased concentrations of PM2.5 in air and accumulating evidences suggesting of their adverse effects in human through airway, it is important and of interesting to evaluate the potential biological adverse effects of PM2.5 collected from air samples in different area. Several factors, such as their effects on cell growth, ROS generation, and cell apoptosis are evaluated. In addition, the biological effects of nanoparticles used for environmental remediation will also be analyzed. Our results have suggested that synthesized schwertmannite (SHM) nanomaterials are bio-compatible and not significantly toxic to mammalian cells, implying they could be reliably be used in environment remediation.

Nanoparticles, cytotoxicity, cell impedance measurement, PM2.5