EPA-104-E3S4-02-02

104

1,500 千元

2015/03/24

2015/12/31

張淑芬

環檢所

陳怡如

知本生技有限公司

水質保護

不對稱流場場流，多角度靜態光散射，環境奈米粒子

Asymmetric Flow Field-Flow-Fractionation (AF4)、Multi-Angle Light Scattering (MALS)、Nanoparticle

陳開憲

張淑芬

02-82278822#

wp4713@ms8.hinet.net

本計畫旨在建置發展本土之水體中奈米物質分離與特性研究分析系統與方法。建置完成之系統包括：不對稱流場場流分離、偵測及分液收集系統，其功能在於分離水體水樣中之奈米粒子並量測其粒徑。粒徑由小自大依序分離至分液收集系統。分液收集後之水樣分別以熱裂解氣相層析質譜儀進行有機成分分析；感應耦合電漿質譜儀進行重金屬含量分析。在不對稱流場場流分離方法開發上分別以PES膜及RC膜交叉輔以不同之移動相:水+200ppm NaN3、1 mM NH4NO3+200ppm NaN3、10 mM NH4NO3+200ppm NaN3、 0.05%SDS+200ppm NaN3分離並分析三種水體水樣(環境檢驗所 前左側汙水蓄水池水樣、高雄地區水庫水樣及環境檢驗所大樓內 自來水)，水庫水樣及自來水水樣因奈米粒子濃度過低，無法量 測其奈米粒徑，無法進行後續分析及討論：汙水蓄水池分離分析 結果：RC膜分析結果RSD變動範圍明顯小於PES膜，PES膜有 明顯自聚及吸附現象分析，RC膜分析結果無此現象，此外離子 強度愈高之流動相，奈米粒子餘分離匣道中之移動速度較慢，對 於小奈米粒子部份，分離效果較佳，整體圖譜圖形釋出時間次序 依序為水相<1mM NH4NO3 <10mM NH4NO3。總體而論，RC膜於本次研究樣品下，其適用性優於PES膜，不對稱流場場流分離系統建置完成，以選用RC膜及10 mM NH4NO3 + 200ppmNaN3流動相可獲得較佳分離效果，未來研究方向須要擴大水體研究數量，以進一步確認目前分離/分析方法的適用性。

不對稱流場場流，多角度靜態光散射，環境奈米粒子

期末報告定稿.pdf

4MB

2016/7/1 上午 12:00:00

The main purpose of this project is to develop separation and identification analytical method for nanoparticle in water. During this study, the well installed and running system include: Asymmetrical Field-Flow Fractionation System、Detection System And Fraction Collection System. The overall function is to separate and identify the nanoparticle via size from small to big and be collected after fractionation. The fractionated sample would be analyzed through Pyrolysis-GCMS and ICP-MS to acquire the composition information on organic compound and heavy metal. The nanoparticle separation analytical method developing is to evaluate performance difference between the two membrane, PES and RC which will run with four mobile phase including：water+200ppm NaN3、1 mM NH4NO3+200ppm NaN3、10 mM NH4NO3+200ppm NaN3、0.05%SDS+200ppm NaN3. The tested samples are three：wastewater effluent from local research institute、reservoir water and tap water. The nanoparticle size on both reservoir water and tap water could not be detected due to the low concentraction. The analysis result of waste water showed that RC had beter RSD than PES, PES had selfaggregation and adsorption on its surface but RC had none of it. Besides, the higher intensity of ion strength, the lower moving speed for all the nanoparticle during separation. This would be help to separate the small size of nanoparticle. The overall required elution time is as following. Water <1mM NH4NO3 <10mM NH4NO3 In conclusion, with the sampes we studied, RC is better than PES to be considered as separation membrane. The whole system of Asymmetrical Field-Flow Fractionation is well established and the analysis ethid is proved and could be used to separate and identify nanoparticle in water. The best combination of membrance and mobile phase would be10 mM NH4NO3 + 200ppm. For the future, it is necessary to increasing the tested samples from different kinds of water body to check the applicability of developed Asymmetrical Field-Flow Fractionation method.

Asymmetric Flow Field-Flow-Fractionation (AF4)、Multi-Angle Light Scattering (MALS)、Nanoparticle