EPA-105-E3S4-02-01

105

1,780 千元

2016/02/24

2016/12/31

林維炤

環檢所

鄧名志

嘉南藥理大學

環境監測及檢測

攪拌子吸附萃取，分散式液液微萃取，藥物

Stir Bar Sorption Extraction (SBSE )，Dispersive Liquid-Liqud Microextraction (DLLME)，Drugs

葉錦芬

林維炤

06-2664911#2422

radical891025@gmail.com

本計畫中以評估攪拌子吸附萃取（Stir Bar Sorption Extraction，SBSE）與分散式液液微萃取（Dispersive Liquid-Liqud Microextraction，DLLME）等微萃取技術應用於水中醫療用藥。本研究計畫所包含:氟希定(Fluoxetine)、氯非安明(Chlorpheniramine)、舍曲林(Sertraline)、甲基安非他命(Methamphetamine)、二苯安明(Diphenhydramine)、二苯環庚丙胺(Amitriptyline)、利度卡因(Lidocaine)、文拉法辛(Venlafaxine)、息達普林(Citalopram)、氯丙噻(Chlorpromazine)、汎泊密(Verapamil)、普帕西芬(Propoxyphene)、普美苯噻(Promethazine)、二氮平(Diazepam)、哌替啶(Meperidine)、美沙酮(Methadone)、多西拉敏(Doxylamine)、米達沙賓(Mirtazapine)、右美沙芬(Dextromethorphan)、可待因(Codeine)共計20種醫療用藥與濫用藥物。 研究結果顯示，以SBSE研究方法進行萃取效率評估:以pH值至約pH=9.2鹼性環境下進行吸附萃取，大部分待測物回收率超過70%，而甲基安非他命(Methamphetamine)、利度卡因(Lidocaine)仍回收率偏低，另外對於可待因(Codeine)待測物無法以攪拌子進行吸附萃取；以DLLME研究方法進行萃取效率評估：以甲苯當萃取溶劑搭配輔助渦漩乳化且不添加鹽類具有較好的萃取回收率，其範圍16.7%~119.0%，然而其中甲基安非他命(Methamphetamine)、普帕西芬(Propoxyphene)、可待因(Codeine)、二氮平(Diazepam)萃取回收率差，其餘待測物皆有85%以上。 經前述兩種前處理技術進行萃取效率評估，結果顯示，SBSE方法對於水中醫療用藥與濫用藥物類具有良好的萃取效率。真實樣品來源於台南市立醫院、台南安平污水處理廠及高雄楠梓污水處理廠之放流水，以SBSE優化條件進行萃取評估。在真實樣品中於台南市立醫院採集之放流水樣品中，檢測出Lidocaine(利度卡因)，其濃度為0.03215 ng/µL，樣品重複(n=2) RSD為0.39%；安平污水處理廠及楠梓污水處理廠中皆未檢出20項醫療用藥與濫用藥物。其基質添加回收率結果顯示本研究待測物台南市立醫院放流水之回收率為13.7 ~ 267.1%，相對標準偏差為5.5 ~ 47.1%；於安平污水處理廠放流水之回收率為12.0 ~ 210.5%，相對標準偏差為0.7 ~ 24.1%；於楠梓污水處理廠放流水之回收率為9.7 ~ 127.9%，相對標準偏差為2.0 ~ 43.0%。 彙整前三年的化合物分為:農藥類與非農藥類，並以攪拌子吸附萃取法進行分析10種不同來源的水樣。對於農藥類以雙攪拌子進行基質評析，其添加回收率有110種農藥回收率皆大於70%；非農藥類(揮發性化合物與極性化合物、半揮發性化合物與酚類化合物、塑化劑、人工合成麝香、防曬劑)受到基質干擾其添加回收率與再現性不佳。

攪拌子吸附萃取，分散式液液微萃取，藥物

EPA-105-E3S4-02-01.pdf

11MB

2017/2/1 上午 12:00:00

This project is the forth years of the microextraction technology evalution for environmental sample. There are two parts in this project. The first is to investigate the application of microextraction methods for medical treatment drugs. The second is to integrate the previous three result and evaluate the applicability of previous result for 10 real samples with different sources by stir bar sorptive extraction. We select Stir Bar Sorptive Extraction (SBSE) and Dispersive Liquid-Liqud Microextraction (DLLME) as the extraction and pretrement methods for the environmental samples. In the first part, twenty medical treatment drugs including fluoxetine、chlorpheniramine、sertraline、methamphetamine、diphenhydramine、amitriptyline、lidocaine、venlafaxine、citalopram、chlorpromazine、verapamil、propoxyphene、promethazine、diazepam、meperidine、methadone、doxylamine、mirtazapine、Dextromethorphan、codeine, were selected as the target compounds. The result showed the recoveries of most of these compounds are larger than 70% at pH9.2 but lower recoveries for methamphetamine and lidocaine by SBSE. Codeine can not be detected by this method. The recoveries ranged from 16.7%~119.0% by vortex DLLME method when toluene used as extraction solvent. The recoveries were poor for methamphetamine、propoxyphene、codeine and diazepam. The recoveries are larger then 85% for other compounds. To evaluate the applicability of previous results. The compounds were divided into two types; pesticide and non-pesticide types. Ten different real samples were tested by dual stir bars SBSE method. For these pesticides, the recoveries of 110 compounds larger then 70%. The result for non-pesticide compounds is poor showed the recoveries were effected by the real sample intereferences.

Stir Bar Sorption Extraction (SBSE )，Dispersive Liquid-Liqud Microextraction (DLLME)，Drugs