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專案計畫編號	EPA-106-E3S5-02-03
經費年度	106
計畫經費	2,100 千元
專案開始日期	2017/03/11
專案結束日期	2017/12/31
專案主持人	童心欣
主辦單位	環檢所
承辦人	林哲雄
執行單位	國立臺灣大學
專案分類	環境監測及檢測
中文關鍵字	硫酸銅，底泥，微生物菌群分布
英文關鍵字	copper sulfate, sediment, microbial community profile
協同主持人
共同主持人	于昌平；郭獻文
計畫聯絡人	周鈺錩
計畫聯絡電話	02-33664404#
計畫聯絡信箱	gold84double@gmail.com

中文摘要	本研究探討以微生物菌群變化作為污染源鑑識之可行性。研究方法分為模擬河川反應槽及現地樣本分析。以河水添加不同濃度硫酸銅連續暴露底泥的方式，定時採取反應槽進出流水樣及底泥樣品，檢測其水樣及底泥菌群變化。現地樣品比較則是採取老街溪底泥及河水樣品，分析其水樣化學特性及底泥生物相。底泥反應槽部分，取用老街溪河水並添加硫酸銅作為進流，定時採取進出流分析，並於第0, 2, 4, 8週取底泥樣品檢測微生物菌群變化。現地樣品部分，由老街溪上游至下游總共採取了13個底泥樣品，19個河水樣品分析。所有的水樣及底泥其核酸進行次世代定序。結果顯示，Aeromonadaceae, Burkholderiales_incertae_sedis, Caldilineaceae, Chloroflexi, Firmicutes, Planctomycetaceae, Pseudomonodaceae, 及Xanthobacteraceae 皆重複出現在現場溪中底泥、溪水、及工廠放流水具有高濃度金屬銅的樣品中，有機會成為銅污染之普遍性指標判斷菌群。另外，由反應槽、老街溪河水及底泥菌相及樣品銅濃度交叉比對，結果顯示，Burkholderiaceae, Hydrogenophilaceae, Methylophilaceae, Chitinophagaceae, 及 Flavobacteriaceae等之豐富度消長與銅濃度相關。未來建議增加現地試驗驗證後可利用其消長變化作為鑑識污染來源的指標。
中文關鍵字	硫酸銅，底泥，微生物菌群分布

中文摘要

本研究探討以微生物菌群變化作為污染源鑑識之可行性。研究方法分為模擬河川反應槽及現地樣本分析。以河水添加不同濃度硫酸銅連續暴露底泥的方式，定時採取反應槽進出流水樣及底泥樣品，檢測其水樣及底泥菌群變化。現地樣品比較則是採取老街溪底泥及河水樣品，分析其水樣化學特性及底泥生物相。底泥反應槽部分，取用老街溪河水並添加硫酸銅作為進流，定時採取進出流分析，並於第0, 2, 4, 8週取底泥樣品檢測微生物菌群變化。現地樣品部分，由老街溪上游至下游總共採取了13個底泥樣品，19個河水樣品分析。所有的水樣及底泥其核酸進行次世代定序。結果顯示，Aeromonadaceae, Burkholderiales_incertae_sedis, Caldilineaceae, Chloroflexi, Firmicutes, Planctomycetaceae, Pseudomonodaceae, 及Xanthobacteraceae 皆重複出現在現場溪中底泥、溪水、及工廠放流水具有高濃度金屬銅的樣品中，有機會成為銅污染之普遍性指標判斷菌群。另外，由反應槽、老街溪河水及底泥菌相及樣品銅濃度交叉比對，結果顯示，Burkholderiaceae, Hydrogenophilaceae, Methylophilaceae, Chitinophagaceae, 及 Flavobacteriaceae等之豐富度消長與銅濃度相關。未來建議增加現地試驗驗證後可利用其消長變化作為鑑識污染來源的指標。

中文關鍵字

硫酸銅，底泥，微生物菌群分布

類型	檔名	檔案大小	說明	最新版公開日期
期末報告	以菌群分布作為鑑識污染源技術建立期末報告(公開版).pdf	4MB	以菌群分布作為鑑識污染源技術建立(1/2)	2018/12/29 上午 12:00:00

摘要	This study investigates the possibility of applying microbial community profile changes to pollution source identification. Due to the time constraint on this project, two directions were deployed simultaneously. One is a laboratory scale sediment microcosm monitoring, the other is field investigation. In the sediment microcosm, river water spiked with different concentrations of copper sulfate was used as the influent and cultured for 8 weeks. Water samples (influents and effluents) were collected periodically, and sediments were collected at 0, 2nd, 4th, and 8th weeks. Field sampling were performed 3 times up to date. Chemical parameters such as nitrate, phosphate, and heavy metal concentrations were analyzed for all water samples from both reactor and field samples. And, crude nucleic acids were also extracted, cleaned and sent for 16S r-RNA gene amplicon sequencing by next generation sequencing with MiSeq platform (Illumina). The results showed that copper sulfate may accumulate in the reactor sediments and may reduce nitrification processes. For the field study, water samples show increasing trends of chloride in both water and sediments from river upstream to downstream. And, high concentrations of metals were observed in several sampling sites along the river. The microbial sequence results from the sediment microcosms, river sediments, river waters, and factory effluents showed that Aeromonadaceae, Burkholderiales_incertae_sedis, Caldilineaceae, Chloroflexi, Firmicutes, Planctomycetaceae, Pseudomonodaceae, and Xanthobacteraceae were observed in all samples and were correlated with copper concentration in CCA analysis. In addition, the abundance of Burkholderiaceae, Hydrogenophilaceae, Methylophilaceae, Chitinophagaceae, and Flavobacteriaceae from were correlated with copper sulfate exposures in microcosm sediments. With proper field testing to verify their repeatability and reproducibility, these families were possible candidates for copper pollution source tracking in rivers or streams.
英文關鍵字	copper sulfate, sediment, microbial community profile

摘要

This study investigates the possibility of applying microbial community profile changes to pollution source identification. Due to the time constraint on this project, two directions were deployed simultaneously. One is a laboratory scale sediment microcosm monitoring, the other is field investigation. In the sediment microcosm, river water spiked with different concentrations of copper sulfate was used as the influent and cultured for 8 weeks. Water samples (influents and effluents) were collected periodically, and sediments were collected at 0, 2nd, 4th, and 8th weeks. Field sampling were performed 3 times up to date. Chemical parameters such as nitrate, phosphate, and heavy metal concentrations were analyzed for all water samples from both reactor and field samples. And, crude nucleic acids were also extracted, cleaned and sent for 16S r-RNA gene amplicon sequencing by next generation sequencing with MiSeq platform (Illumina). The results showed that copper sulfate may accumulate in the reactor sediments and may reduce nitrification processes. For the field study, water samples show increasing trends of chloride in both water and sediments from river upstream to downstream. And, high concentrations of metals were observed in several sampling sites along the river. The microbial sequence results from the sediment microcosms, river sediments, river waters, and factory effluents showed that Aeromonadaceae, Burkholderiales_incertae_sedis, Caldilineaceae, Chloroflexi, Firmicutes, Planctomycetaceae, Pseudomonodaceae, and Xanthobacteraceae were observed in all samples and were correlated with copper concentration in CCA analysis. In addition, the abundance of Burkholderiaceae, Hydrogenophilaceae, Methylophilaceae, Chitinophagaceae, and Flavobacteriaceae from were correlated with copper sulfate exposures in microcosm sediments. With proper field testing to verify their repeatability and reproducibility, these families were possible candidates for copper pollution source tracking in rivers or streams.

英文關鍵字

copper sulfate, sediment, microbial community profile