EPA-106-E3S4-02-03

106

1,400 千元

2017/04/10

2017/12/31

張淑芬

環檢所

金孝義

知本生技有限公司

環境監測及檢測

不對稱流場場流，多角度靜態光散射，環境奈米粒子

Asymmetric Flow Field-Flow-Fractionation (AF4)、Multi-Angle static Light Scattering (MALS)、Environmental Nanoparticle

張瑜玲

02-82278822#

tinachang@systematic.com.tw

本期計畫欲發展建構適用於生物毒性檢測之環境水體奈米物質分離技術。共採取十八件水樣：都市廢水、鈑金噴漆加工業、機械加工區、科學園區、造紙業、陶瓷業、化妝品原料製造業、電子業、皮革加工製造業、光電業及自然水體。 利用動態光散射儀初步篩選水體中之奈米物質，評估是否進入不對稱流場場流系統分離，並評析動態光散射儀與不對稱流場場流分離系統間之關聯性；檢測水體以不對稱流場場流分離分析系統研究水樣中奈米粒子尺寸分布，再以熱裂解氣相層析質譜儀及感應耦合電漿質譜儀進行有機成分/無機金屬之組成狀態。同時提供水中奈米粒子分離技術方法草案。 前處理0.1 µm濾膜條件下，十八件水樣有十件水樣之動態光散射偵測值高於初篩標準；而於前處理0.22 µm濾膜條件下，十八件水樣有十二件水樣之偵測值高於初篩標準。多數水樣於水移動相條件下，不對稱流場場流系統皆可成功由小至大分離水樣中之粒子；樣品再現性佳，部分樣品因不同樣品瓶而有些許訊號強度的差異，但不影響粒徑分離分布趨勢。而十件水樣中除造紙業放流水外，ρ值(Rg/Rh)皆大於1，可見目前測試水樣中之奈米粒子多數為結構較鬆散的型態；而造紙業放流水ρ值為0.6 ~ 0.8，構型接近實心之圓球體。 利用感應耦合電漿質譜儀(ICP-MS)，委託檢驗單位進行分析，針對砷(As)、鎘(Cd)、鉻(Cr)、銅(Cu)、鉛(Pb)、銀(Ag)、鋅(Zn)、鐵(Fe)八個重金屬；另額外分析錳(Mn) / 鎳(Ni) / 硒(Se) / 鈣(Ca) / 鎂(Mg)，共十三種金屬/元素成分。其中檢驗出砷(As)、銅(Cu)、鋅(Zn)、鐵(Fe)、錳(Mn)、鎳(Ni)、鈣(Ca)及鎂(Mg)。 此外也檢測總有機碳Total Organic Carbon (TOC)、水中揮發性有機化合物(Volatile Organic Compound, VOC)及以熱裂解氣相層析質譜儀進行有機組成分析。 配合之細胞實驗室針對都市廢水、鈑金噴漆加工業、機械加工區共六件水樣進行生物毒性試驗，觀察到鈑金噴漆加工業進流水樣影響細胞存活率，配合粒徑分析及有機/無機成分組成推估造成細胞存活率下降之因素，達到發展適用於生物毒性檢測之環境水體奈米物質分離技術的目的。

不對稱流場場流，多角度靜態光散射，環境奈米粒子

3-3 期末報告 定稿本 .pdf

7MB

2018/2/1 上午 12:00:00

The plan is to develop the nanoparticles separation technology for biotoxicity testing in environmental water. A total of eighteen water samples were taken: municipal wastewater, paper industry, mechanical processing park, sheet metal painting industry, semiconductor science park, ceramics, cosmetics raw materials manufacturing, electronic industry, leather processing and manufacturing, optical electronics industry and natural water. The separation of the asymmetric flow field field (AF4) system was carried out after the dynamic light scattering (DLS) instrument screen the nanoparticles in the water. The correlation between the DLS and the AF4 separation system was evaluated. AF4 separation system was used to study the size distribution of nanoparticles in water samples. The composition of organic components / inorganic metals was also studied by pyrolysis gas chromatography mass spectrometry (GC-MS) / inductively coupled plasma mass spectrometer (ICP-MS), and providing the draft method for separation of nanoparticles in water. The DLS detection of 10 in 18 water samples is higher than the screening standard in 0.1µm filter. The detected value of 12 samples is higher than the screening standard for 0.22µm filter. Most of the samples, the asymmetric flow field field (AF4) system separates successfully from small to large particles in water mobile phase and good reproducibility. Different vials might be signal differences but does not affect the distribution of particle size. Theρ(Rg / Rh) value greater than 1 shows that the nanoparticles of these water samples are loose structure except for Paper industry effluent. Theρvalue of Paper industry effluent 0.6 to 0.8 indicates the structure close to the solid sphere. Aarsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), lead (Pb), silver (Ag), zinc (Zn), iron (Fe) are analyzed by inductively coupled plasma mass spectrometry (ICP-MS) and are also manganese (Mn) / nickel (Ni) / selenium (Se) / calcium (Ca) / magnesium (Mg), a total of thirteen kinds of metal / elemental components. As, Cu, Zn, Fe, Mn, Ni, Ca and Mg are tested among these water samples. Total Organic Carbon (TOC), Volatile Organic Compound (VOC) in water and organic composition analysis by pyrolysis gas chromatography mass spectrometry were also done. A total of six water samples of municipal wastewater, sheet metal painting industry and mechanical processing park are tested for biological toxicity. The original water of sheet metal painting industry affects the cell viability. We could estimate the cause of cells survival rate decline with the particle size and organic / inorganic components analysis and achieve the development of nano-material separation technology for biological toxicity testing in environmental water.

Asymmetric Flow Field-Flow-Fractionation (AF4)、Multi-Angle static Light Scattering (MALS)、Environmental Nanoparticle