環保專案成果報告查詢系統

專案計畫編號	EPA-106-1603-02-01
經費年度	106
計畫經費	2,364 千元
專案開始日期	2017/01/25
專案結束日期	2017/12/31
專案主持人	王尚禮
主辦單位	環檢所
承辦人	陳明妮
執行單位	國立臺灣大學
專案分類	土壤及地下水
中文關鍵字	重金屬、雷射剝蝕感應耦合電漿質譜法、元素空間分布
英文關鍵字	Heavy metals, Laser ablation inductively coupled plasma mass spectrometry, Elemental mapping
協同主持人	葉國禎
共同主持人	葉國楨
計畫聯絡人	林家維
計畫聯絡電話	02-33664808#
計畫聯絡信箱	cwlin@ntu.edu.tw

中文摘要	本計畫發展土壤與植物體中重金屬濃度及空間分布的LA-ICP-MS分析方法，以及X光吸收光譜分析重金屬物種型態之方法，以期了解決定土壤重金屬物種和空間分布的關鍵因子和反應，以及重金屬被植物吸收和傳輸過程的機制。以LA-ICP-MS分析土壤和植體標準品和樣品之結果顯示，LA-ICP-MS分析砷、鎘、鉻、銅、汞、鎳、鉛、鋅的測值和ICP-MS的結果接近，且利用內標法可以部分提升分析的表現，但準確度仍有待提升。此外，結果顯示LA-ICP-MS具有穩定的分析再現性，此確保其應用於土壤和植體元素空間分布分析的可行性。在本計畫中進而運用此方法測定稻米和水稻根部土壤的重金屬空間分布，分析稻米和水稻根重金屬分布，並利用X光吸收光譜和序列萃取法分析重金屬之物種型態。所獲得的結果顯示這些方法於分析土壤和植體內，以及植物根/土壤界面的重金屬物種和空間分布的可行性，以探究決定重金屬物種和空間分布的關鍵土壤反應，進而探討重金屬轉變和傳輸的機制。
中文關鍵字	重金屬、雷射剝蝕感應耦合電漿質譜法、元素空間分布

中文摘要

本計畫發展土壤與植物體中重金屬濃度及空間分布的LA-ICP-MS分析方法，以及X光吸收光譜分析重金屬物種型態之方法，以期了解決定土壤重金屬物種和空間分布的關鍵因子和反應，以及重金屬被植物吸收和傳輸過程的機制。以LA-ICP-MS分析土壤和植體標準品和樣品之結果顯示，LA-ICP-MS分析砷、鎘、鉻、銅、汞、鎳、鉛、鋅的測值和ICP-MS的結果接近，且利用內標法可以部分提升分析的表現，但準確度仍有待提升。此外，結果顯示LA-ICP-MS具有穩定的分析再現性，此確保其應用於土壤和植體元素空間分布分析的可行性。在本計畫中進而運用此方法測定稻米和水稻根部土壤的重金屬空間分布，分析稻米和水稻根重金屬分布，並利用X光吸收光譜和序列萃取法分析重金屬之物種型態。所獲得的結果顯示這些方法於分析土壤和植體內，以及植物根/土壤界面的重金屬物種和空間分布的可行性，以探究決定重金屬物種和空間分布的關鍵土壤反應，進而探討重金屬轉變和傳輸的機制。

中文關鍵字

重金屬、雷射剝蝕感應耦合電漿質譜法、元素空間分布

類型	檔名	檔案大小	說明	最新版公開日期
期末報告	2017-環檢所-期末報告_定稿本-公開版.pdf	19MB		2018/2/1 上午 12:00:00

摘要	To decipher the complex reactions controlling the bioavailability of heavy metals in soils, one of the important tasks is the understanding of the key reactions and factors that determine the speciation and spatial distribution of heavy metals in soils and the acquisition and translocation of heavy metals in plants. Thus, this study aimed at developming LA-ICP-MS method in conjunction with X-ray absorption (XAS) method for determining the speciation and spatial distribution of heavy metals in soils and plants. This work evaluated the accuracy, precision, sensitivity and detection limit of the methods in determining the contents of As, Cd, Cr, Cu, Hg, Ni, Pb and Zn in soil and plant samples and certified materials, and the feasibility and analytical performace of XAS in determining the speciations of those heavy metals in soil and plant samples. The results obtained by LA-ICP-MS were comparable to the counterparts of solution ICP-MS analysis in terms of accuracy and precision. To further improve the performance of the method, different quantitation strategies were employed. The results revealed that the appropriate selection of internal standard can improve the analytical performance of the LA-ICP-MS method in the analysis of As, Cd, Cr, Cu, Hg, Ni, Pb and Zn in soil and plant samples. Meanwhile, the good reproducibity of the LA-ICP-MS method indicated the feasibility of applying the method in determining the spatial distribution of heavy metals in soil and plant samples. The synergetic application of the LA-ICP-MS and synchrotron-based XAS methods provides the possibility in observing the distribution and transformation of metals within a heterogeneous sample, such as soils and plants, and at soil-plant root interfaces. The information was applied to investigate the key processes that determine the bioavailability and toxicity of heavy metals in soils and their transformation within the plants.
英文關鍵字	Heavy metals, Laser ablation inductively coupled plasma mass spectrometry, Elemental mapping

摘要

To decipher the complex reactions controlling the bioavailability of heavy metals in soils, one of the important tasks is the understanding of the key reactions and factors that determine the speciation and spatial distribution of heavy metals in soils and the acquisition and translocation of heavy metals in plants. Thus, this study aimed at developming LA-ICP-MS method in conjunction with X-ray absorption (XAS) method for determining the speciation and spatial distribution of heavy metals in soils and plants. This work evaluated the accuracy, precision, sensitivity and detection limit of the methods in determining the contents of As, Cd, Cr, Cu, Hg, Ni, Pb and Zn in soil and plant samples and certified materials, and the feasibility and analytical performace of XAS in determining the speciations of those heavy metals in soil and plant samples. The results obtained by LA-ICP-MS were comparable to the counterparts of solution ICP-MS analysis in terms of accuracy and precision. To further improve the performance of the method, different quantitation strategies were employed. The results revealed that the appropriate selection of internal standard can improve the analytical performance of the LA-ICP-MS method in the analysis of As, Cd, Cr, Cu, Hg, Ni, Pb and Zn in soil and plant samples. Meanwhile, the good reproducibity of the LA-ICP-MS method indicated the feasibility of applying the method in determining the spatial distribution of heavy metals in soil and plant samples. The synergetic application of the LA-ICP-MS and synchrotron-based XAS methods provides the possibility in observing the distribution and transformation of metals within a heterogeneous sample, such as soils and plants, and at soil-plant root interfaces. The information was applied to investigate the key processes that determine the bioavailability and toxicity of heavy metals in soils and their transformation within the plants.

英文關鍵字

Heavy metals, Laser ablation inductively coupled plasma mass spectrometry, Elemental mapping