EPA-107-1605-02-01

107

2,210 千元

2018/02/23

2018/12/31

謝季吟

環檢所

李秋萍

國立屏東科技大學

科技計畫

端足類、搖蚊、底泥品質指標

Amphipods, Chironomid, Sediment Quality Guidelines

王一匡

謝季吟

08-7703202#7507

chiying@mail.npust.edu.tw

本計畫參考歐盟相關底泥生物毒性測試方法，建置搖蚊(Chironomus riparius)底泥生物毒性檢測技術，並與現行公告之端足蟲 (Hyalella azteca) 進行參考毒物之靈敏度比較。 C. riparius已可穩定於實驗室中馴養及繁殖。毒性試驗之精密度試驗結果顯示，C. riparius暴露於KCl之48h-LC50 值介於2.287～2.744 g/L，CV值為6.9 %顯示再現性高。H. azteca 及C. riparius 試驗前健康度評估均低於生物試驗之CV值50% 規範。參考毒物試驗結果顯示 H. azteca 暴露於重金屬Cd及有機物fluoranthene之96小時LC50平均值分別為6.26±0.28μg/L與35.39±2.03μg/L； C. riparius 暴露於Cd與fluoranthene之48小時LC50平均值分別為5.66±0.65μg/L與 82.28±2.75 μg/L。野外全底泥TIE毒性鑑定評估結果顯示，樣站HJ1、CGB及CGC在添加粉狀椰子炭後顯著降低其毒性，顯示主要致毒性物質可能為PAHs 化合物；而孔隙水毒性鑑定評估結果顯示，樣站CGA在添加EDTA後會顯著降低其毒性，顯示樣站主要導致毒性物質可能為重金屬。整體而言，環境底泥及孔隙水樣品中不同類別的化合物(有機和無機)和毒性之間存在相關性，但這些相關性並不代表兩者和毒性之間存在著直接的因果關係，其它可能存在於底泥中有機碳或孔隙水萃取流程改變底泥動態平衡都可能是影響毒性的因子。本計畫運用底棲無脊椎生物進行底泥品質指標適宜性結果顯示，端足蟲及搖蚊對重金屬及有機污染物之靈敏度可評估底泥品質之優劣，未來可搭配化學分析結果以有效詮釋底泥污染物之生物有效性，提供未來作為之參酌。

端足類、搖蚊、底泥品質指標

EPA-107-1605-02-01期末報告 .pdf

8MB

期末報告

2019/2/1 上午 12:00:00

Chironomus riparius sediment toxicity testing based on the European Union's relevant sediment toxicity test protocol was carried out and its suitability was assessed. The sensitivity of Chironomus riparius was then compared to Hyalella azteca by evaluating two standard reference toxicants, Cd and fluoranthene A commonly used toxicological species, C. riparius, has been successfully acclimatized in the laboratory. The toxicological responses of reference toxicant KCl obtained by acute (48 h test) LC50 values for C. riparius ranged from 2.287 to 2.744 g/L, and the CV value was 6.9%. These results were highly reproducible. A pre-test determination of the health of H. azteca and C. riparius test specimens was consistent with the recommended CV values being below 50%. In metal and organic compound toxicity tests, the acute (96 h) LC50 values of Cd and fluoranthene for H. azteca were 6.26±0.28 μg/L and 35.39±2.03μg/L, respectively. The acute (48 h) LC50 values for C. riparius were 5.66±0.65 μg/L for Cd and 82.28±2.75 μg/L for fluoranthene. The TIE results for whole sediment showed that the main toxicants at sites HJ1, CGB, and CGC were PAH compounds due to significantly reduced toxicity after the addition of coconut charcoal. Porewater toxicity was significantly reduced after the addition of EDTA, showing that the main toxicant was trace metal at site CGA. Overall, TIE can be used to confirm that a causative toxicant has been correctly identified and quantitatively correlate its toxicity to the measured concentration. This study revealed that different (organic or inorganic) compounds showed specific toxicities in whole sediment and porewater toxicity tests; however, other confounding factors such as chemicals or organic carbons that change the dynamic equilibrium defy toxicity identification. Using benthic invertebrate organisms for evaluating guidelines for sediment quality showed that the sensitivity of the amphipods H. azteca and C. riparius to trace metals and organic compounds were sufficient to assess sediment quality. In the future, such benthic bioassay should be combined with chemical analysis to provide biological risk assessments that include bioavailability of toxicants of contaminated sediments for regulatory pratice

Amphipods, Chironomid, Sediment Quality Guidelines