環保專案成果報告查詢系統

專案計畫編號
經費年度	112
計畫經費	3,050 千元
預算科目	化學物質評估與管理-化學物質登錄審查
專案開始日期	2023/05/08
專案結束日期	2023/11/30
專案主持人	羅宇軒
主辦單位	化學署評估管理組
承辦人	管庭冬
執行單位	國立臺灣大學
專案分類	化學物質管理
中文關鍵字	轉錄體學、關鍵特徵法、生殖危害
英文關鍵字	Transcriptomics, key characteristics, reproductive hazard
協同主持人
共同主持人
計畫聯絡人	羅宇軒
計畫聯絡電話	02-33668087#
計畫聯絡信箱	ysluo@ntu.edu.tw

中文摘要	由於高階毒理終點取得人物力成本較高，因而化學物質標準登錄實務上常缺乏高階毒理資料。本計畫利用關鍵特徵法綜整致癌與生殖危害物的機制資料，結合系統性文獻回顧與轉錄體學資訊，以協助填補化學物質潛在的毒理資料缺口。本計畫從關鍵特徵法與細胞轉錄體學資料出發，提出生殖危害物質的細胞轉錄體學關鍵特徵，並建立初期評估化學物質生殖危害特性的工作流程。本計畫使用已知生殖危害物質壬基酚、8種壬基酚衍生物與3種人工甜味劑作為個案研究，以驗證此工作流程的可靠性。本計畫取得受測物質於乳癌細胞株MCF-7內的細胞轉錄體學資料，進行生物調控路徑分析，並與本計畫第一階段所建立的生殖危害預警生物調控路徑相比對，研析化學物質所引起的生殖危害關鍵特徵，並據以評估化學物質具生殖危害的可能性。
中文關鍵字	轉錄體學、關鍵特徵法、生殖危害

中文摘要

由於高階毒理終點取得人物力成本較高，因而化學物質標準登錄實務上常缺乏高階毒理資料。本計畫利用關鍵特徵法綜整致癌與生殖危害物的機制資料，結合系統性文獻回顧與轉錄體學資訊，以協助填補化學物質潛在的毒理資料缺口。本計畫從關鍵特徵法與細胞轉錄體學資料出發，提出生殖危害物質的細胞轉錄體學關鍵特徵，並建立初期評估化學物質生殖危害特性的工作流程。本計畫使用已知生殖危害物質壬基酚、8種壬基酚衍生物與3種人工甜味劑作為個案研究，以驗證此工作流程的可靠性。本計畫取得受測物質於乳癌細胞株MCF-7內的細胞轉錄體學資料，進行生物調控路徑分析，並與本計畫第一階段所建立的生殖危害預警生物調控路徑相比對，研析化學物質所引起的生殖危害關鍵特徵，並據以評估化學物質具生殖危害的可能性。

中文關鍵字

轉錄體學、關鍵特徵法、生殖危害

類型	檔名	檔案大小	說明	最新版公開日期
期末報告	化學物質關鍵特徵法分析應用計畫_期末報告_1121219_修正3.pdf	12MB		2024/2/24 上午 12:00:00

摘要	Due to the higher cost and demanding efforts, chemicals often lack higher-tier toxicological data in practicing standard chemical registration. This project utilizes the key characteristic approach to synthesize mechanistic data for carcinogens and reproductive hazards, combining systematic literature reviews with transcriptomic information to help fill potential toxicological data gaps for chemicals. Combining the key characteristic approach and cell transcriptomic data, this project proposes the key characteristics of the cellular transcriptome for reproductive toxicants and establishes an initial workflow to assess the reproductive hazard of chemicals. The project incorporates known reproductive hazard substance nonylphenol, eight nonylphenol derivatives, and three artificial sweeteners in a case study to verify the reliability of this workflow. We acquire the transcriptomic data of the test substances within the breast cancer cell line MCF-7, perform biological pathway analysis, compare the pathway analysis results with the alerting pathways of reproductive hazard established in the first phase of this project, analyze the chemical-induced key characteristics for reproductive hazard, and then determine whether the test substance carries the potential reproductive hazards of chemicals.
英文關鍵字	Transcriptomics, key characteristics, reproductive hazard

摘要

Due to the higher cost and demanding efforts, chemicals often lack higher-tier toxicological data in practicing standard chemical registration. This project utilizes the key characteristic approach to synthesize mechanistic data for carcinogens and reproductive hazards, combining systematic literature reviews with transcriptomic information to help fill potential toxicological data gaps for chemicals. Combining the key characteristic approach and cell transcriptomic data, this project proposes the key characteristics of the cellular transcriptome for reproductive toxicants and establishes an initial workflow to assess the reproductive hazard of chemicals. The project incorporates known reproductive hazard substance nonylphenol, eight nonylphenol derivatives, and three artificial sweeteners in a case study to verify the reliability of this workflow. We acquire the transcriptomic data of the test substances within the breast cancer cell line MCF-7, perform biological pathway analysis, compare the pathway analysis results with the alerting pathways of reproductive hazard established in the first phase of this project, analyze the chemical-induced key characteristics for reproductive hazard, and then determine whether the test substance carries the potential reproductive hazards of chemicals.

英文關鍵字

Transcriptomics, key characteristics, reproductive hazard