環保專案成果報告查詢系統

專案計畫編號
經費年度	113
計畫經費	3,350 千元
預算科目	化學物質科學研究-化學物質科學研究
專案開始日期	2024/03/22
專案結束日期	2024/11/30
專案主持人	羅宇軒
主辦單位	化學署評估管理組
承辦人	崔君至
執行單位	國立臺灣大學
專案分類	化學物質管理
中文關鍵字	轉錄體學、代謝體學、生殖危害
英文關鍵字	Transcriptomics, Metabolomics, Reproductive hazard
協同主持人
共同主持人
計畫聯絡人	葉于嘉
計畫聯絡電話	33668087
計畫聯絡信箱	yjyeh1206@ntu.edu.tw

中文摘要	隨著科技進展，化學品種類日益增加，卻僅有少數化學物質具備完整毒理資料。考量動物福祉、時間經濟成本以及國際趨勢，開發替代測試方法以減少實驗動物使用是當前重要之課題。本計畫接續前期計畫發展之轉錄體學關鍵特徵法，以乳癌細胞株MCF-7多濃度測試資料的量化資訊，進一步最佳化生殖毒性篩選方法的靈敏度與特異度，並以壬基酚及其衍生物作為個案研究，以驗證篩選方法可靠性。此外，初步評估細胞外代謝體分析資料是否可進一步提升篩選方法。最後，為瞭解可用於調查國人對於化學物質潛在暴露狀況的技術，蒐研化學物質暴露評估之新興採樣方法，並探討其在我國運用之可行性，及評估可優先運用對象與情境。本計畫以81種物質細胞轉錄體學資料所建立的生殖危害特性篩選方法，其靈敏度達88%，特異度達56%，未來可依據國際最新研究，進一步擴充本方法的化學物質應用域，並提升生殖危害物質篩選方法的可靠性。
中文關鍵字	轉錄體學、代謝體學、生殖危害

中文摘要

隨著科技進展，化學品種類日益增加，卻僅有少數化學物質具備完整毒理資料。考量動物福祉、時間經濟成本以及國際趨勢，開發替代測試方法以減少實驗動物使用是當前重要之課題。本計畫接續前期計畫發展之轉錄體學關鍵特徵法，以乳癌細胞株MCF-7多濃度測試資料的量化資訊，進一步最佳化生殖毒性篩選方法的靈敏度與特異度，並以壬基酚及其衍生物作為個案研究，以驗證篩選方法可靠性。此外，初步評估細胞外代謝體分析資料是否可進一步提升篩選方法。最後，為瞭解可用於調查國人對於化學物質潛在暴露狀況的技術，蒐研化學物質暴露評估之新興採樣方法，並探討其在我國運用之可行性，及評估可優先運用對象與情境。本計畫以81種物質細胞轉錄體學資料所建立的生殖危害特性篩選方法，其靈敏度達88%，特異度達56%，未來可依據國際最新研究，進一步擴充本方法的化學物質應用域，並提升生殖危害物質篩選方法的可靠性。

中文關鍵字

轉錄體學、代謝體學、生殖危害

類型	檔名	檔案大小	說明	最新版公開日期
期末報告	物質潛在危害之生物標記分析應用計畫_成果報告.pdf	9MB		2025/1/6 上午 12:00:00

摘要	With advancements in technology, the diversity of chemical substances has increased rapidly; however, comprehensive toxicological data are available for only a limited subset of these chemicals. In light of concerns surrounding animal welfare, as well as the need for time- and cost-efficient approaches, developing new approach methodologies (NAMs) to minimize the use of laboratory animals has become a pressing priority. This project builds upon a previously established transcriptomics-based Key Characteristics (KCs) method to optimize the sensitivity and specificity of reproductive toxicity screening. Using quantitative data from multi-concentration assays on the MCF-7 breast cancer cell line, the reliability of the screening method was validated through case studies on nonylphenol and its derivatives. Furthermore, this study investigated whether the inclusion of extracellular metabolomic data could enhance the performance of the screening method. Finally, to explore technologies applicable for investigating the potential chemical exposure scenarios among the population, research will focus on emerging sampling methods for chemical exposure assessment. Additionally, the feasibility of applying these methods in Taiwan will be examined, along with outlining the priority targets and contexts for their implementation. The transcriptomics-based reproductive toxicity screening method, developed using data from 81 chemicals, demonstrated a sensitivity of 88% and a specificity of 56%. Future work will aim to expand the chemical applicability domain of this method by incorporating insights from the latest international research, thereby improving its reliability and utility in identifying reproductive toxicants.
英文關鍵字	Transcriptomics, Metabolomics, Reproductive hazard

摘要

With advancements in technology, the diversity of chemical substances has increased rapidly; however, comprehensive toxicological data are available for only a limited subset of these chemicals. In light of concerns surrounding animal welfare, as well as the need for time- and cost-efficient approaches, developing new approach methodologies (NAMs) to minimize the use of laboratory animals has become a pressing priority. This project builds upon a previously established transcriptomics-based Key Characteristics (KCs) method to optimize the sensitivity and specificity of reproductive toxicity screening. Using quantitative data from multi-concentration assays on the MCF-7 breast cancer cell line, the reliability of the screening method was validated through case studies on nonylphenol and its derivatives. Furthermore, this study investigated whether the inclusion of extracellular metabolomic data could enhance the performance of the screening method. Finally, to explore technologies applicable for investigating the potential chemical exposure scenarios among the population, research will focus on emerging sampling methods for chemical exposure assessment. Additionally, the feasibility of applying these methods in Taiwan will be examined, along with outlining the priority targets and contexts for their implementation. The transcriptomics-based reproductive toxicity screening method, developed using data from 81 chemicals, demonstrated a sensitivity of 88% and a specificity of 56%. Future work will aim to expand the chemical applicability domain of this method by incorporating insights from the latest international research, thereby improving its reliability and utility in identifying reproductive toxicants.

英文關鍵字

Transcriptomics, Metabolomics, Reproductive hazard