環保專案成果報告查詢系統

專案計畫編號	EPA-92-E3S5-02-01
經費年度	092
計畫經費	1,425 千元
專案開始日期	2003/02/01
專案結束日期	2003/12/31
專案主持人	許美芳
主辦單位	環檢所
承辦人
執行單位	環工系許美芳, 環管所陳庭堅, 藥學系陳福安
專案分類	環境監測及檢測
中文關鍵字	壬基酚, 三丁基錫, 風險評估, 雌激素效應, 雄激素效應
英文關鍵字	Nonylphenol, Tribulytin, Risk Assessment,,Estrogenic Effect, Androgenic Effect
協同主持人	謝季吟
共同主持人	陳庭堅, 陳福安
計畫聯絡人	許美芳
計畫聯絡電話	08-7624002分機407
計畫聯絡信箱	mfshue@ccsun.tajen.edu.tw

中文摘要	依今年度計畫工作目標之要求分化學分析及生物效應分析等兩部分，化學檢測部分除於高屏溪流域及沿海採集真實環境檢體水樣及底泥樣品外，需另加民生用品之部分進行壬基酚或三丁基錫的含量分析，壬基酚項目共完成之樣品數：溪流水樣44個、地下水20個，底泥10個，民生用品中洗滌劑4件、乳化劑3件，總共81個樣品；三丁基錫項目共完成之樣品數：沿海水樣20個，底泥6個，魚肉9件，漁網4件總共39個樣品。綜合化學分析工作總樣品數共120個，超過原定之工作目標。壬基酚項目在溪流樣品、底泥、乳化劑檢出率皆100 %，水樣測值介0.19~183.4mg/l，底泥測值介134.7-354.4 ng/g，乳化劑測值介1.9-13.4- mg/g，部分測站水樣壬基酚測值高於國內外文獻資料，底泥測值則略低於國內北部地區的數據。地下水樣品壬基酚與類雄激素效應的檢出率皆為0 %。三丁基錫項目水樣檢出率25 %、底泥檢出率83.3 %，水樣測值介＜0.0026~83.2 ng/l，底泥測值介＜1.24~72.39 ng/g，，以汕尾漁港的測值明顯高於其他測站。魚肉樣品檢出率100 % ，測值介26.4~194.2 ng/g；此三類樣品的測值與文獻資料差異不大，另廢棄漁網的檢出率0 %，未見三丁基錫的殘留。 84個樣品以兩種生物效應檢測技術：一為利用人類乳癌細胞株 MVLN雌激素專一性轉錄分析法，探討雌激素效應物質之篩選，另一為採用MCF7-AR1細胞，探討雄激素效應物質之篩選，並於高屏溪流域採集84個樣品，進行真實環境樣品之檢測，驗證了此兩種生物分析法，應用在環境議題上的可行性。原水樣雌激素效應分析，依Soto分類系統顯示樣品中屬full agonist的佔4.8 ﹪，partial agonist的佔7.1 ﹪；反觀雄激素效應則partial agonist的佔4.8 ﹪但無full agonist，但若經濃縮處理後則雌、雄激素效應都明顯上升，因此水體品質之安全性需進行長期監控。今年度對壬基酚與三丁基錫兩物種的風險評估，也提出一些資料，也依實際環境檢體之測值與國際組織所設定的預期無效應濃度(PNEC)作生態風險評估，對人類族群則採用MOS（安全限值，估計無效應濃度/暴露濃度）。整體而言今年度，水樣及底泥NP的含量對水中生物及底棲生物可能造成潛在風險；至於TBT部分尚須更多的檢體以確定對水生生物的潛在風險。
中文關鍵字	壬基酚, 三丁基錫, 風險評估, 雌激素效應, 雄激素效應

中文摘要

依今年度計畫工作目標之要求分化學分析及生物效應分析等兩部分，化學檢測部分除於高屏溪流域及沿海採集真實環境檢體水樣及底泥樣品外，需另加民生用品之部分進行壬基酚或三丁基錫的含量分析，壬基酚項目共完成之樣品數：溪流水樣44個、地下水20個，底泥10個，民生用品中洗滌劑4件、乳化劑3件，總共81個樣品；三丁基錫項目共完成之樣品數：沿海水樣20個，底泥6個，魚肉9件，漁網4件總共39個樣品。綜合化學分析工作總樣品數共120個，超過原定之工作目標。壬基酚項目在溪流樣品、底泥、乳化劑檢出率皆100 %，水樣測值介0.19~183.4mg/l，底泥測值介134.7-354.4 ng/g，乳化劑測值介1.9-13.4- mg/g，部分測站水樣壬基酚測值高於國內外文獻資料，底泥測值則略低於國內北部地區的數據。地下水樣品壬基酚與類雄激素效應的檢出率皆為0 %。三丁基錫項目水樣檢出率25 %、底泥檢出率83.3 %，水樣測值介＜0.0026~83.2 ng/l，底泥測值介＜1.24~72.39 ng/g，，以汕尾漁港的測值明顯高於其他測站。魚肉樣品檢出率100 % ，測值介26.4~194.2 ng/g；此三類樣品的測值與文獻資料差異不大，另廢棄漁網的檢出率0 %，未見三丁基錫的殘留。 84個樣品以兩種生物效應檢測技術：一為利用人類乳癌細胞株 MVLN雌激素專一性轉錄分析法，探討雌激素效應物質之篩選，另一為採用MCF7-AR1細胞，探討雄激素效應物質之篩選，並於高屏溪流域採集84個樣品，進行真實環境樣品之檢測，驗證了此兩種生物分析法，應用在環境議題上的可行性。原水樣雌激素效應分析，依Soto分類系統顯示樣品中屬full agonist的佔4.8 ﹪，partial agonist的佔7.1 ﹪；反觀雄激素效應則partial agonist的佔4.8 ﹪但無full agonist，但若經濃縮處理後則雌、雄激素效應都明顯上升，因此水體品質之安全性需進行長期監控。今年度對壬基酚與三丁基錫兩物種的風險評估，也提出一些資料，也依實際環境檢體之測值與國際組織所設定的預期無效應濃度(PNEC)作生態風險評估，對人類族群則採用MOS（安全限值，估計無效應濃度/暴露濃度）。整體而言今年度，水樣及底泥NP的含量對水中生物及底棲生物可能造成潛在風險；至於TBT部分尚須更多的檢體以確定對水生生物的潛在風險。

中文關鍵字

壬基酚, 三丁基錫, 風險評估, 雌激素效應, 雄激素效應

類型	檔名	檔案大小	說明	最新版公開日期
期末報告	0000012062.zip	131MB	[期末報告]	2004/3/1 上午 12:00:00

摘要	The analyses were distinguished by chemical analysis and bioassay. Water and sediment samples were taken along Kaoping Rive and estuary. Fractions of household commodities were also analyzed for Nonylphenol (NP) or tribulytin (TBT). The sample sizes totaled 81 for NP test, 44 for river water, 20 for groundwater, 10 for sediment, 4 for household commodities, and 3 for emulsion respectively. The sample sizes totaled 39 for TBT test, 20 for seawater, 6 for sediment, 9 for fish muscular, and 4 for fishnet, respectively. NP concentration for water samples, sediment, and emulsion agent were 0.19~183.4 mg/l, 134.7-354.4 ng/g, and 1.9-13.4 mg/g, respectively. The TBT analytic results showed that 25% of the samples contained TBT. Samples for sediment contained 83.3% of TBT. The concentration of TBT for water samples and sediment were ＜0.0026~83.2 ng/l，and ＜1.24~72.39 ng/g. The TBT concentration in Fish Port of Shann-Wei was much greater than other sampling locations. Samples of fish muscular contained 100% of TBT residuary that reached a concentration between 26.4 and 194.2 ng/g. Bioassay MVLN investigated estrogenic activity substance of the samples. MCF-AR1 examined androgen-like substance. 84 samples taken from Kaoping Rive were analyzed by both bioassay methods. The results form estrogenic activity analysis showed that 4.8% of the samples belonged to full agonist and 7.1% of the samples belonged to partial agonist. As for androgen activity analysis, it showed that 4.8% of samples belonged to partial agonist and none of samples belonged to full agonist. Both NP and TBT were begun on risk assessment. The measured concentration was divided by Predicted No-Effect Concentration (PNEC) to obtain ecotoxicity hazard quotient. Human risk characterization was evaluated for Margin of Safety (MOS) that accepted daily intake/measured daily intake. The results of risk characterization showed that the levels of NP in both water and sediment might cause potential risks to aquatic and benthonic organisms. However, more samples were needed to verify potential TBT risk.
英文關鍵字	Nonylphenol, Tribulytin, Risk Assessment,,Estrogenic Effect, Androgenic Effect

摘要

The analyses were distinguished by chemical analysis and bioassay. Water and sediment samples were taken along Kaoping Rive and estuary. Fractions of household commodities were also analyzed for Nonylphenol (NP) or tribulytin (TBT). The sample sizes totaled 81 for NP test, 44 for river water, 20 for groundwater, 10 for sediment, 4 for household commodities, and 3 for emulsion respectively. The sample sizes totaled 39 for TBT test, 20 for seawater, 6 for sediment, 9 for fish muscular, and 4 for fishnet, respectively. NP concentration for water samples, sediment, and emulsion agent were 0.19~183.4 mg/l, 134.7-354.4 ng/g, and 1.9-13.4 mg/g, respectively. The TBT analytic results showed that 25% of the samples contained TBT. Samples for sediment contained 83.3% of TBT. The concentration of TBT for water samples and sediment were ＜0.0026~83.2 ng/l，and ＜1.24~72.39 ng/g. The TBT concentration in Fish Port of Shann-Wei was much greater than other sampling locations. Samples of fish muscular contained 100% of TBT residuary that reached a concentration between 26.4 and 194.2 ng/g. Bioassay MVLN investigated estrogenic activity substance of the samples. MCF-AR1 examined androgen-like substance. 84 samples taken from Kaoping Rive were analyzed by both bioassay methods. The results form estrogenic activity analysis showed that 4.8% of the samples belonged to full agonist and 7.1% of the samples belonged to partial agonist. As for androgen activity analysis, it showed that 4.8% of samples belonged to partial agonist and none of samples belonged to full agonist. Both NP and TBT were begun on risk assessment. The measured concentration was divided by Predicted No-Effect Concentration (PNEC) to obtain ecotoxicity hazard quotient. Human risk characterization was evaluated for Margin of Safety (MOS) that accepted daily intake/measured daily intake. The results of risk characterization showed that the levels of NP in both water and sediment might cause potential risks to aquatic and benthonic organisms. However, more samples were needed to verify potential TBT risk.

英文關鍵字

Nonylphenol, Tribulytin, Risk Assessment,,Estrogenic Effect, Androgenic Effect