So far, the study has obtained some results listed below: 1. The components of the optimal medium for the strain FJ03 are NaNO3 0.5 g/l, K2HPO4 0.06 g/l, MgSO4•7H2O 2.0 g/l, CaCl2•2H2O 0.06 g/l, citric acid 0.06 g/l, ferric ammonium citrate 0.25 g/l, EDTA 0.001 g/l, Na2CO3 0.02 g/l and trace metal solution 1 ml. 2. The concentrations of CO2 for strain FJ03 with greater growth is 10%. 3. Parameters of kinetic model for the growth of strain FJ03: growth coefficient (Y) 0.035 mg-cell/mg-CO3-2, specific growth rate (m) 0.186 day-1, specific substrate utilization (k) 5.31 mg-CO3-2/mg VSS-day, death coefficient (b) 0.0032 day-1, Monod half saturation coefficient (Ks) 9.08 mg-CO3-2/L. 4. In a 2 liter-continuous-culture system of strain FJ03, the biomass was able to maintain in 0.7 of the OD600 value with setting the flow rate of nutrient addition and algal culture efferent on 19 ml/hour. 5. The project has successfully developed a novel 200-liter photo-bioreactor for biological fixation of CO2. The nutrient addition and algal culture efferent with the flow rate of 8.34 L/h can stably keep the production of strain FJ 03 with 0.7 of OD600 value in the 200-liter photo-bioreactor by continuous production model. 6. Using the continuous cultivation model, the fixation efficiency of CO2 by strain FJ03 was 0.371 g-CO2/L/day, and the productivity of strain FJ03 is 0.221 g-cell/L/day under the operation conditions of 0.7 of biomass OD600, and 10% CO2 with sparging rate of 0.616 L/sec.

bioreactor, carbon dioxide fixation, microalgae