| 英文摘要 |
Fungi exist in both indoors and outdoors. Exposure to fungi, particularly in water damaged indoor environments, has been thought to exacerbate a number of adverse health effects, ranging from subjective symptoms such as fatigue, cognitive difficulties or memory loss to more definable diseases such as allergy, asthma and hypersensitivity pneumonitis. Traditionally, fungi have been recovered by plate cultivation. Fungal identification may require days to weeks and expertise is needed for reliably identification. Recently, DNA microarray technology displays a high potential in the testing and analysis of environmental samples. Recent applications of the DNA microarray in environment studies include the detection of pathogens, environmentally functional genes, specific bacteria and viruses. In this study, based on the sequences of the ITS regions, an oligonucleotide array was developed to identify fungal pathogens in air. A collection of 73 target strains (21 species) were analyzed by the array and a sensitivity of 98.6% (72/73) was obtained. In addition, a total of 66 nontargrt strains (66 species) were also tested and a specificity of 100% (66/66) was obtained. Furthermore, 143 isolates from air samples were analyzed by the array; the correct identification rate was 100% (71/71) and the specificity was 98.6% (71/72). The present method is a rapid and accurate alternative for identification of fungi isolated from air samples.
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