環境中奈米物質量測、特性分析及即時毒性測試平台技術開發
| 中文摘要 | 為模擬人體肺部沈積奈米微粒的情況，本研究使用一新設計之奈米濕式靜電微粒採樣器(Wet Nanoparticle Sampler, WNS)來採集大氣或實驗室製備之奈米微粒，取得的奈米水樣再以本研究自行設計之奈米微粒細胞暴露靜電沉積器(Cell ESP)進行微粒與細胞的接觸，其中Cell ESP係根據電泳沉積之理論而設計的。本研究利用細胞連續及即時生長分析儀，直接偵測暴露過奈米微粒細胞的生長情形，進而推估奈米微粒毒性效應(與中興大學闕斌如教授合作)。結合WNS及Cell ESP即為本研究所開發之奈米微粒細胞毒行測試平台。 透過前三期的努力，本團隊已能準確地採集環境奈米微粒並分析其主要化學成分，且有好的微粒化學質量平衡。相關研究成果已發表4篇學術論文於國際知名的Atmospheric Environment及Aerosol Science and Technology期刊。本期研究以先前的研究成果為基礎，繼續選擇一般民眾、學生、駕駛乘客、遊客及作業勞工或工廠附近居民容易長時間暴露奈米微粒的地方及一個工廠使用奈米碳黑的作業場所，探究人體可能暴露奈米微粒的情形。本期另於鹿林山環保署背景測站及雪隧進、出口進行奈米物質物化特性等相關的研究，期能歸納出東亞生質燃燒遠程傳輸現象、大氣核化作用及SOA生成機制及隧道外居民的交通奈米暴露情形等。 本研究已完成「奈米物質量測分析技術與暴露評估」有關之最新資訊整理(critical review)文件，內容包括氣懸及水中的奈米物質，完整的報告後將至少在環保署「環境奈米科技知識庫」中具名發表，以供各界參考。 | ||
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| 中文關鍵字 | 奈米微粒採樣及化學分析;奈米微粒質量平衡;奈米物質環境安全衛生;奈米微粒細胞毒性測試平台;PAHs固氣分離器 | ||
基本資訊
| 專案計畫編號 | EPA-99-U1U1-02-103 | 經費年度 | 099 | 計畫經費 | 5100 千元 |
|---|---|---|---|---|---|
| 專案開始日期 | 2010/04/16 | 專案結束日期 | 2010/12/31 | 專案主持人 | 蔡春進 |
| 主辦單位 | 永續發展室(停用) | 承辦人 | 蘇鈺珊 | 執行單位 | 交通大學 |
成果下載
| 類型 | 檔名 | 檔案大小 | 說明 |
|---|---|---|---|
| 期末報告 | EPA99U1U102103.pdf | 6MB |
Developing measurement, characterization and cellular toxicity testing platform techniuqes for envi
| 英文摘要 | In order to simulate the nanoparticle deposition in the human lung, this study developed an electrostatic Wet Nanoparticle Sampler (WNS) to collect atmospheric and laboratory produced nanoparticles. Electric filed was then apply to deposit nanoparticles suspended in the liquid on the pre-cultured cell by a newly developed Cell Electrostatic Precipitator (Cell-ESP) based on the electrophoretic theory. Finally, the cell index of the exposed cell lines was measured by a real-time cell growth analyzer to determine the nanotoxicity (in cooperation with Prof. Chueh of Chung Hsing University). The combination of the WNS and Cell-ESP is the nanoparticle cell toxicity testing platform developed in this study. As the results of this study, we demonstrated that the samples of environmental nanoparticle could be collected accurately and good chemical mass closure (CMC) could be obtained. The research results were published in four journal papers in world renowned Atmospheric Environment and Aerosol Science and Technology. This year, we continued to conduct the samplings at the locations where city residents, students, drivers, tourists as well as workers were likely to expose to nanoparticles and at a workplace where nano-BC was used. In addition, the characteristatics of nanoparticles were studied at the LuLin background site and inside and at the outlet of SyueShan tunnel. It is expected that the long rang transport of air pollutants from biomass burning in Southeast Asia, and nucleation and formation of SOA (secondary organic aerosol) can be better understood, and the exposoure levels of the residents to engine nanoparticles can be quantified. This study also reviewed the latest literature on the measurement and characterization methods for environmental nanomaterials in the air and water. The summary report will be submitted to the environmental nanotechnology knowledge plateform of Taiwan EPA for publication and public use. | ||
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| 英文關鍵字 | nanoparticle sampling and analysis;mass closure of nanoparticles;EHS of nanomaterials;nanotoxicity;PAHs denuder | ||