| 英文摘要 |
Nanomaterials have received considerable recent attention because of their unique properties and diverse applications in technology and life sciences. However, the nano-structures of these materials possess very different physical and chemical properties compared to their larger size counterparts. Given the increasing use of nanoparticles in a range of products, it has become crucial to develop a fundamental understanding of the biological effects of nanoparticle exposure. Many in vitro studies have suggested that nanoparticles are cytotoxic and genotoxic; however, results are inconsistent, mainly due to interference between nanoparticles and dye molecules commonly used in traditional cytotoxicity assays. The purpose of this study was to establish and validate an in vitro cytotoxicity screening platform for evaluating biological effects of nanoscale zero-valent iron. In addition, the characterization of nanoparticles will be examined by TEM/SEM. Moreover, the effect of PM2.5 particles, fine particles 2.5 micrometers or less in size will be examined using the cell impedance measurement and flow cytometry.
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