土壤中持久性污染物對微生物族群變異及生物復育基因評析資訊建置
| 中文摘要 | 由於持久性有機污染物的生物難分解特性，當其進入土壤後造成環境污染影響甚巨。瞭解土壤中的微生物族群結構及其對持久性污染物之分解代謝利用，對於污染土壤的生物復育極為重要。本計畫以高通量次世代定序技術（Next-Generation Sequencing），分析受多氯聯苯及五氯酚污染之土壤環境中微生物族群結構變化及篩選並分析多氯聯苯及五氯酚分解代謝之菌株的轉錄體。結果發現五氯酚污染後會使得變形菌門族群增加3倍；多氯聯苯污染會使得變形菌門族群增加1.3倍及放線菌門族群增加1.4倍。由beta多樣性分析及heat map分析瞭解到不同土壤中的微生物族群結構不因受到相同污染而趨同，仍能保持其來源特異性，可應用於環境法醫參考。轉錄體分析2株多氯聯苯分解菌及1株五氯酚分解菌雖然未能直接發現相關代謝基因，但仍有許多表顯差異極大的基因可作為候選生物標識。 | ||
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| 中文關鍵字 | 次世代定序、多氯聯苯、五氯酚 | ||
基本資訊
| 專案計畫編號 | EPA-104-1605-02-01 | 經費年度 | 104 | 計畫經費 | 1960 千元 |
|---|---|---|---|---|---|
| 專案開始日期 | 2015/03/27 | 專案結束日期 | 2015/12/31 | 專案主持人 | 黃介辰 |
| 主辦單位 | 環檢所 | 承辦人 | 林哲雄 | 執行單位 | 國立中興大學 |
成果下載
| 類型 | 檔名 | 檔案大小 | 說明 |
|---|---|---|---|
| 期末報告 | EPA10416050201.pdf | 70MB |
Evaluation of Bioremediation Gene and Microorganism Community Diversity in POPs Contaminated Soil
| 英文摘要 | Persistent organic pollutants contamination is concered because it is hard to biodegrade. Understanding the structure of the soil microbial communities and the metabolic pathway of POPs for the bioremediation of contaminated soil is extremely important. We apply next-generation sequencing technology for analysis of microorganism diversity changes and transcriptomics of biodegraded bacteria in polychlorinated biphenyls or pentachlorophenol contaminated soil. It was found that would make the three-fold increase Proteobacteria after pentachlorophenol contamination. PCB contamination will cause an increase of 1.3 times in Proteobacteria and 1.4 times in Actinobacteria. The microorganism community is still different between two soils even when they undergo the same pollutant by the beta diversity and heat map analysis. The specifity of microorganism community can further applied in environmental forensics. Analysis of two PCBs degraded bacteria and a PCP degraded bacteria, though not directly related to metabolic genes found, but there are still a number of transcricpts with very different expression can be used as candidate biomarkers. | ||
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| 英文關鍵字 | Next-generation sequencing, Polychlorinated biphenyls, Pentachlorophenol | ||