開發雷射剝蝕感應耦合電漿質譜法應用於土壤及植物體中重金屬之傳輸研究（1/3）
| 中文摘要 | 本計畫發展土壤與植物體中重金屬濃度及空間分布的LA-ICP-MS分析方法，並配合同步輻射的微光譜法來分析樣品特定區域的重金屬物種型態，以期了解決定土壤重金屬物種和空間分布的關鍵因子和反應，以及重金屬被植物吸收和傳輸過程的機制。以LA-ICP-MS分析土壤和植體標準品和樣品之結果顯示，LA-ICP-MS分析砷、銅和鋅的測值和ICP-MS的結果接近，且利用內標法可以部分提升分析的表現。此外，結果顯示LA-ICP-MS具有穩定的分析再現性，此確保其應用於土壤和植體元素空間分布分析的可行性。在本計畫中進而運用此方法測定稻米和水稻根部土壤的重金屬空間分布，同時結合同步輻射的µ-XRF 和µ-XAS方法，分析稻米和水稻根重金屬分布，以及特定熱區的砷物種型態。所獲得的結果顯示這些方法於分析土壤和植體內，以及植物根/土壤界面的重金屬物種和空間分布的可行性，以探究決定重金屬物種和空間分布的關鍵土壤反應，進而探討重金屬轉變和傳輸的機制。 | ||
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| 中文關鍵字 | 重金屬，雷射剝蝕感應耦合電漿質譜法，元素空間分布 | ||
基本資訊
| 專案計畫編號 | EPA-105-1603-02-01 | 經費年度 | 105 | 計畫經費 | 2460 千元 |
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| 專案開始日期 | 2016/03/30 | 專案結束日期 | 2016/12/31 | 專案主持人 | 王尚禮 |
| 主辦單位 | 環檢所 | 承辦人 | 陳明妮 | 執行單位 | 國立臺灣大學 |
成果下載
| 類型 | 檔名 | 檔案大小 | 說明 |
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| 期末報告 | 2016-環檢所-期末報告-定稿本-公開版.pdf | 9MB |
Development of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) method for the study of heavy metals transport in soil and plant
| 英文摘要 | To decipher the complex reactions controlling the bioavailability of heavy metals in soils, one of the important tasks is the understanding of the key reactions and factors that determine the speciation and spatial distribution of heavy metals in soils and the acquisition and translocation of heavy metals in plants. Thus, this proposed study aims at developing LA-ICP-MS method in conjunction with µ-XRF and µ-XAS methods for determining the speciation and spatial distribution of heavy metals in soils and plants. As, Cd, Cu and Zn were analyzed on soil and plant samples and certified materials. The results of As, Cd and Zn obtained by LA-ICP-MS were shown to be comparable to the counterparts of solution ICP-MS analysis in terms of accuracy and precision. To further improve the performance of the method, different quantitation strategies were employed. The results revealed that the appropriate selection of internal standard can improve the analytical performance of the method. Meanwhile, the good reproducibility of the LA-ICP-MS method indicates the feasibility of applying the method in determining the spatial distribution of heavy metals in soil and plant samples, which was demonstrated on rice grain and rhizosphere soil samples. The synergetic application of the LA-ICP-MS and synchrotron-based µ-XRF and µ-XAS methods provides the possibility in observing the distribution and transformation of metals within a heterogeneous sample, such as soils and plants, and at soil-plant root interfaces. The information will advance our understanding of the key processes that determine the bioavailability and toxicity of heavy metals in soils and their transformation within the plants after metals are absorbed by the plants. | ||
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| 英文關鍵字 | Heavy metals,Laser ablation inductively coupled plasma mass spectrometry, Elemental mapping | ||