| 英文摘要 |
With the increasing awareness of ethical issues and animal protection, scientists have begun seeking more humane and accurate alternative methods. Due to the continuous advancement in in vitro cell and tissue culture technologies, in vitro tissue culture is considered a significant technique that can replace traditional animal testing. Among these, the development of in vitro three-dimensional (3D) cell culture technology is believed to meet the need for alternatives to traditional animal experiments. The 3D cell culture technique simulates a cell environment that is closer to in vivo conditions, allowing for a more accurate reflection of the effects of chemicals on the human body. As a result, it has become increasingly important in toxicity testing and drug development, not only reducing the reliance on animal models but also improving the reliability and reproducibility of experimental results. In this project, the research team developed microfluidic cell culture devices to quickly and easily form large quantities of size-uniform 3D liver tissues using human and mouse liver cell lines. The experiment developed a comprehensive cell culture and 3D liver tissue formation and culture procedure. Using these 3D tissues, chemical cytotoxicity tests can be evaluated. Additionally, the experiment also developed genotoxicity testing protocols, including micronucleus and comet assays. Preliminary results confirmed the feasibility of replacing animal testing with these developed methods. In the experiments, the in vitro test data are compared with existing animal test data using analytical methods to provide alternative testing approaches for translating animal test results. It is hoped that the demand for animal testing will be significantly reduced in the near future.
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