運用分子生物技術提升厭氧好氧薄膜濾離生物反應器(MBR)之生物分解功能
| 中文摘要 | TFT-LCD光電業製程廢水殘留未完全反應之剝離劑(如DMSO、MEA或BDG)、顯影劑(如TMAH)、表面處理劑及溶劑(如IPA)等，其有機碳，有機氮及有機硫之化學特性複雜，直接影響厭氧生物可分解率(Biodegradability)。在本研究中，採取國內三家大型LCD廢水處理場之厭氧污泥及好氧污泥，自AO process，應用厭氧酸化產氫潛能試驗(BHP test)，厭氧甲烷化潛能(BMP test)，缺氧脫硝產氮潛能(BNP test)，以及好氧比攝氧速率(S.OUR test)，等批分式生物分解試驗，測試各種功能菌類之生物活性，得以比較各廢水場現有污泥中產氫菌、甲烷化菌、脫硝菌以及硝化菌之生物活性。並應用掃描式電子顯微鏡，觀測厭氧污泥及好氧污泥之微生物社會結構及菌體型態，得以建立各廢水處理場生態型態指標。其中以MBR膜離生物反應槽截留多樣性菌體族群，最為顯著。並在本研究中將T-RFLP (Terminal restriction fragment length polymorphism)分生檢測技術應用於偵測光電廢水廢水處理系統，可以定性分辨光電廢水污泥中之厭氧產氫菌、厭氧甲烷化菌、缺氧脫硝菌以及好氧硝化菌。尤其改良開發正逆雙向基因序列之修飾限制酶檢測法，可以正確鑑定氨氧化菌(AOB)及亞硝酸氧化菌(NOB)之種屬分類，已達定性標準。應用T-RFLP快速分生檢測技術，可在現場採樣兩天內完成五種功能性菌類之種屬鑑定，進而提供各種菌種之生物指標，以及時調控兩段生物程序，並提升生物分解功能。康那香企業公司開發兩組不織布薄膜濾離模組，可用於厭氧生物反應槽(Anoxic MBR)，以及好氧生物反應槽(Oxic MBR)之程序組合，並有效處理光電廢水中有機碳、有機氮以及氨氮。不織布膜離生物反應槽(Non-Woven MBR)模組設計改良，已可以套裝設備方式產品化，康那香企業公司已具有製造及組裝實績，並發展為環保設備產業。建議推廣應用到石化業、化工業及食品業並加強模型場研發(Pilot study)，以利工程放大設計。 | ||
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| 中文關鍵字 | TFT-LCD製程廢水,薄膜濾離生物反應器(MBR),尾端限制片段長度多型性分析(T-RFLP),微生物多樣性,,,, | ||
基本資訊
| 專案計畫編號 | EPA-93-U1U4--006 | 經費年度 | 093 | 計畫經費 | 1980 千元 |
|---|---|---|---|---|---|
| 專案開始日期 | 2004/05/01 | 專案結束日期 | 2004/12/31 | 專案主持人 | 鄭幸雄 |
| 主辦單位 | 永續發展室(停用) | 承辦人 | 執行單位 | 國立成功大學 |
成果下載
| 類型 | 檔名 | 檔案大小 | 說明 |
|---|---|---|---|
| 期末報告 | 0000023025.pdf | 0MB | [期末報告]公開版 |
Application of molecular biotechnology to improve biodegradation of anaerobic/aerobic membrane bioreactor
| 英文摘要 | Characterization of the TFT-LCD manufacturing wastewater discharged from three plants was conducted. Relatively high ratio of organic nitrogen to organic carbon conducted from certain amount of stripper (DMSO&MEA) developer (TMAH) and chelating agents. So tow stages of anoxic denitrification and oxic nitrification processes are developed to degrade the specific organic nitrogen and ammonium. Non-woven membrane bioreactors (NW-MBR) are designed and installed with plate-frame modules of Non-woven membrane, so called KNH-MBR.Tow months of continuous flow process of tow-stage KNH-MBR have been studied to evaluate the performance of denitrification and nitrification. The anoxic MBR attended to limited degradation of organic nitrogen and denitrification, while the aerobic MBR achieved good removal efficiency of TOC COD organic nitrogen and ammonium. Extended process operation should be done with acclimated inoculation and enough biomass of denitrifier.Four methods of biodegradation batch test are conducted. The biochemical hydrogen potential (BHP) test, the BMP test, the BNP test and the specific oxygen uptake rate (SOUR) measurement showed the KNH-MBR anaerobic and aerobes degraded TOC, organic nitrogen and ammonium oxidation with nitrifier.The ecological indicators of various function bacteria were observed with the scanning electronic microscopy. Terminal restriction fragment length polymorphism (T-RFLP) was modified and developed for identification of nitrifier and denitrifier in the anoxic sludge and oxic sludge of LCD wastewater treatment plants fingerprint of bacterial species could be expressed from the T.RFLP diagram. Several species of ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) were identified according to the genetic database. So molecular bio monitoring methodology could be conducted to investigate the population dynamics of the existed microbial community in the MBR process. | ||
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| 英文關鍵字 | TFT-LCD wastewater,membrane bioreactor (MBR),Terminal restriction fragment length polymorphism (T-RFLP),microbial diversity | ||