環境水樣中腸病毒檢測技術之建立及地下水、飲用水污染調查
| 中文摘要 | 腸道病毒已被證明和許多經由水體傳播之痢疾案例有關，並引起許多公共衛生上的問題。大約有超過140種以上的腸道病毒會經由糞便排放而污染水體。本計畫之目的，包括評估水體中腸病毒經各種濃縮、流洗方式處理後腸病毒之回收狀況。為評估各種水體病毒的濃縮狀況，我們所選擇的水體包括醫院廢水、水廠原水、水廠清水，以及經調整酸鹼值和鹽類濃度之水體。另外，我們也評估PCR分析過程之各項操作條件，並以定量PCR進行病毒含量之相對定量分析。而經本計畫評估之最佳分析流程，乃用於進行環境水樣腸病毒存在性分析。核酸分析步驟包括：RNA萃取、RT-PCR反應、PCR產物分析以及環境中PCR反應之干擾因子探討。本計畫總共從十三個採樣點採集二十個水樣進行腸病毒分析。其中十四個水樣來自以表面水為水源之水廠的原水及清水，三個來自於以地下水為水源之水廠原水，三個來自於簡易自來水設施。檢測結果顯示，七個表面水水廠原水樣本中，有四個樣本檢測出腸病毒，七個表面水水廠清水樣本中，有五個樣本檢測出腸病毒，三個地下水樣本中，有一個檢測出腸病毒，至於三個簡易自來水水樣則有兩個檢測出腸病毒。而上述樣本進行腸病毒細胞培養之結果，皆呈陰性反應。 | ||
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| 中文關鍵字 | 腸病毒,飲用水,地下水 | ||
基本資訊
| 專案計畫編號 | EPA-93E3S50201 | 經費年度 | 093 | 計畫經費 | 1260 千元 |
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| 專案開始日期 | 2004/02/12 | 專案結束日期 | 2004/12/31 | 專案主持人 | 許昺慕 |
| 主辦單位 | 環檢所 | 承辦人 | 執行單位 |
成果下載
| 類型 | 檔名 | 檔案大小 | 說明 |
|---|---|---|---|
| 期末報告 | 00000416841.pdf | 3MB | [期末報告] |
Development of Enteroviruses Detecting Techniques and Survey of Enteroviruses in the Groundwater and Drinking Water
| 英文摘要 | Enteric viruses have been associated with many outbreaks of waterborne nonbacterial gastroenteritis and are a public health concern. The objective of this project is to evaluate series of procedures to concentrate viruses by adsorption to and elution from various charged membranes for various types of water. To ensure the best possible evaluation for detection of viruses in different kinds of water type samples, the hospital wastewater, raw, treated, and adjusted pHs and electrolyte treated water samples were provided. These concentration procedures were combined with various PCR methods in order to detect enteroviruses in environmental water samples. The relative recovery efficiencies of enterovirus were also evaluated by Real-Time PCR. The optimum procedures designed for enteroviruses detection of the environmental water samples were done in this study. The nucleic acid analyzing method for enteroviruses includes the following steps: RNA extraction, reverse transcription polymerase chain reaction (RT-PCR) and analysis of the PCR-products. The contents of environmental inhibitors after virus concentration are suspected to have some inhibitory effect on viruses PCR detection. It should be considered while detecting enteric viruses in environmental samples. Environmental water samples were taken from thirteen prescreened locations around the island. A total of twenty water samples were obtained during 2 months. Results revealed that four out of seven raw water samples and five out of seven treated water samples from surface water treatment plants were detected positive for enteroviruses. One out of three ground water samples and two out of three small units water samples were detected positive for enteroviruses. It is advised that the Taiwan EPA keeps investigating this related research, so as to scientifically estimate the long-term potential health risk. | ||
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| 英文關鍵字 | Enteroviruses,Drinking water,Groundwater | ||