環保專案成果報告查詢系統

專案計畫編號	EPA-105-GA13-03-A194
經費年度	105
計畫經費	13,800 千元
預算科目	提升土壤及地下水污染調查及整治技術工作
專案開始日期	2016/05/24
專案結束日期	2017/11/23
專案主持人	吳素慧
主辦單位	土污基管會
承辦人	詹皓凱
執行單位	台灣中油股份有限公司探採研究所
專案分類	土壤及地下水
中文關鍵字	環境法醫、市售柴油、生物標誌物、指紋圖譜、診斷比值、主成份分析、檢測技術、氣相層析質譜儀
英文關鍵字	GC/MS, Measuring Technologies, Principal Component Analysis, Diagnostic ratio, Chemical fingerprint chromatogram, Biomarker, Diesel, Environmental forensics
協同主持人
共同主持人
計畫聯絡人	吳素慧
計畫聯絡電話	037-356150#201
計畫聯絡信箱	cclai@epa.gov.tw

中文摘要	本期計畫期末執行成果，依計畫目標與工作內容，分述如下： (一)完成前期計畫所建立的Excel簡易資料庫與Endnotes文獻管理資料庫更新(共計258篇)，並建立關鍵字分類索引。針對與柴油污染洩漏時間鑑識有關的二苯併噻吩族群化合物特性，以及統計分析技術之應用與汽油洩漏污染鑑識技術等相關文獻進行研析。另根據計畫的執行成果，完成國內油污染指紋鑑識技術判釋流程的更新，以及提出加油站的柴油洩漏污染鑑識作業參考程序指引草案。 (二)完成中油與台塑油品合計40個市售高柴樣品GC/MS分析與資料處理，以及市售柴油樣品分析數據資料庫的擴充。並完成彙總3期計畫共307個市售柴油樣品的油品供應源鑑識PCA-DRs群聚分析，成功驗證與強化了前期所建立的市售柴油油品供應源鑑識技術的適用性。 (三)完成土壤採樣品保規畫書撰寫、15處場址篩選名單與土壤採樣規劃書的撰寫、11處污染場址土壤採樣與103個鑑識用樣品篩選。另由業者或儲槽計畫提供9處47個土壤樣品，扣除4重複場址總計取得16處污染場址與150個土壤樣品。 (四)完成16個污染場址106個土壤樣品的萃取、GC/MS分析與資料處理，以及各場址污染油品供應源PCA-DRs群聚分析鑑識技術應用性驗證。並提出分別適用於無汽油干擾與受汽油干擾之PCA-DRs群聚分析的20個與25個診斷比值群組，以及加油站柴油洩漏污染鑑識執行架構與作業參考程序兩個建議流程，完備加油站柴油洩漏污染油品供應源鑑識技術。 (五)運用土壤樣品萃取物中，所含烷基二苯併噻吩族群化合物的相對含量，配合車用柴油硫含量管制標準，進行柴油污染洩漏時序的鑑識技術，本計畫完成16個污染場址的應用案例彙總解析，歸納出目前可遵循的鑑識參考程序。 (六)完成三次鑑識技術講習，參加2017年7月於北京舉辦的2017 International Network of Environmental Forensics (INEF) 研討會，綜合本計畫與前期計畫執行成果論文發表，並分別於國際書籍與國內研討會各發表1篇論文與4篇海報論文。
中文關鍵字	環境法醫、市售柴油、生物標誌物、指紋圖譜、診斷比值、主成份分析、檢測技術、氣相層析質譜儀

中文摘要

本期計畫期末執行成果，依計畫目標與工作內容，分述如下： (一)完成前期計畫所建立的Excel簡易資料庫與Endnotes文獻管理資料庫更新(共計258篇)，並建立關鍵字分類索引。針對與柴油污染洩漏時間鑑識有關的二苯併噻吩族群化合物特性，以及統計分析技術之應用與汽油洩漏污染鑑識技術等相關文獻進行研析。另根據計畫的執行成果，完成國內油污染指紋鑑識技術判釋流程的更新，以及提出加油站的柴油洩漏污染鑑識作業參考程序指引草案。 (二)完成中油與台塑油品合計40個市售高柴樣品GC/MS分析與資料處理，以及市售柴油樣品分析數據資料庫的擴充。並完成彙總3期計畫共307個市售柴油樣品的油品供應源鑑識PCA-DRs群聚分析，成功驗證與強化了前期所建立的市售柴油油品供應源鑑識技術的適用性。 (三)完成土壤採樣品保規畫書撰寫、15處場址篩選名單與土壤採樣規劃書的撰寫、11處污染場址土壤採樣與103個鑑識用樣品篩選。另由業者或儲槽計畫提供9處47個土壤樣品，扣除4重複場址總計取得16處污染場址與150個土壤樣品。 (四)完成16個污染場址106個土壤樣品的萃取、GC/MS分析與資料處理，以及各場址污染油品供應源PCA-DRs群聚分析鑑識技術應用性驗證。並提出分別適用於無汽油干擾與受汽油干擾之PCA-DRs群聚分析的20個與25個診斷比值群組，以及加油站柴油洩漏污染鑑識執行架構與作業參考程序兩個建議流程，完備加油站柴油洩漏污染油品供應源鑑識技術。 (五)運用土壤樣品萃取物中，所含烷基二苯併噻吩族群化合物的相對含量，配合車用柴油硫含量管制標準，進行柴油污染洩漏時序的鑑識技術，本計畫完成16個污染場址的應用案例彙總解析，歸納出目前可遵循的鑑識參考程序。 (六)完成三次鑑識技術講習，參加2017年7月於北京舉辦的2017 International Network of Environmental Forensics (INEF) 研討會，綜合本計畫與前期計畫執行成果論文發表，並分別於國際書籍與國內研討會各發表1篇論文與4篇海報論文。

中文關鍵字

環境法醫、市售柴油、生物標誌物、指紋圖譜、診斷比值、主成份分析、檢測技術、氣相層析質譜儀

類型	檔名	檔案大小	說明	最新版公開日期
期末報告	期末報告定稿本EPA-105-GA13-03-A194(本文)_1061220 - 公開版(上傳).pdf	14MB		2018/5/1 上午 12:00:00

摘要	According to the proposed aims (objectives) and contents in this project, we summarize our accomplishments as follows. The established Excel and Endnotes databases have updated which collected 258 research articles in total corresponding to the oil spill identification, and also created a keyword classification index. These literatures were reviewed to include 1. dibenzothiophenes fingerprinting identification technology for age-dating study; 2. the application of statistics analysis for the classification of the principal component analysis (PCA) of source oils; 3. gasoline spill contaminated identification technology. In overall, based on the results accumulated in this project, we have achieved our goals to renew the operation protocols of the chemical fingerprinting technology for the oil spill identification. Moreover, an operation procedure guideline for the identification of the diesel spill pollution of gas stations has also been drafted. 40 commercialized diesel samples collected from the gas stations of both CPC Co., Taiwan, and Formosa Petroleum Corporation, were acquired for GC/MS analysis and subsequent data process. The information has been updated for the chemical fingerprint database of the commercialized diesels. To gather 307 samples collected since the first stage diesel forensics research project until now, we have further carried out the study of their principal component analysis-diagnostics ratios (PCA-DRs) and been verifying the technology that we have built since last project which is indeed valid for source identification of the commercialized diesel oils. Proposals for quality control of soil collection and identification and characterization of soil samples polluted by diesel spill collected from 15 identified sites have been drafted and illustrated. 11 gas station sites with diesel spill were carried out the soil sample collection and 103 diesel spill soil samples were obtained from these sites. Additional 47 polluted soil samples from 9 diesel spill contaminated sites were also provided by private sector and executors of oil tank survey project. Among them, four polluted sites were actually repeated. Hence, there were 150 samples being obtained from 16 contaminated sites in total. Pretreatment of 106 soil samples from 16 diesel spill contaminated sites were carried out and subsequently acquired by GC/MS analysis and data process. All the PCA-DRs cluster analyses for the classification of the diesel oils from the polluted sites were verified for their applications. We have also provided 20 and 25 diagnostic ratio groups, respectively, for the ones without and with the gasoline interferences. The protocols for source oil identification from the diesel spill sites and their operation procedures were suggested. The source investigation and identification of diesel spills from gasoline stations have been completely established. The relative amount of dibenzothiophene derivatives to C4-naphthalenes in the soil extract can be applied for the age-dating preliminary estimate of diesel spill. This project completes the application case analysis of 16 contaminated sites and summarizes the current reference procedures that can be followed. Three technical seminars for the identification and characterization of the oil spill have been held at EPA. The project leader participated in the International Network of Environmental Forensics (INEF) conference held in Beijing in July 2017, and presented the project performed result. One book chapter was published in English and 4 poster presentations were performed in domestic conferences.
英文關鍵字	GC/MS, Measuring Technologies, Principal Component Analysis, Diagnostic ratio, Chemical fingerprint chromatogram, Biomarker, Diesel, Environmental forensics

摘要

According to the proposed aims (objectives) and contents in this project, we summarize our accomplishments as follows. The established Excel and Endnotes databases have updated which collected 258 research articles in total corresponding to the oil spill identification, and also created a keyword classification index. These literatures were reviewed to include 1. dibenzothiophenes fingerprinting identification technology for age-dating study; 2. the application of statistics analysis for the classification of the principal component analysis (PCA) of source oils; 3. gasoline spill contaminated identification technology. In overall, based on the results accumulated in this project, we have achieved our goals to renew the operation protocols of the chemical fingerprinting technology for the oil spill identification. Moreover, an operation procedure guideline for the identification of the diesel spill pollution of gas stations has also been drafted. 40 commercialized diesel samples collected from the gas stations of both CPC Co., Taiwan, and Formosa Petroleum Corporation, were acquired for GC/MS analysis and subsequent data process. The information has been updated for the chemical fingerprint database of the commercialized diesels. To gather 307 samples collected since the first stage diesel forensics research project until now, we have further carried out the study of their principal component analysis-diagnostics ratios (PCA-DRs) and been verifying the technology that we have built since last project which is indeed valid for source identification of the commercialized diesel oils. Proposals for quality control of soil collection and identification and characterization of soil samples polluted by diesel spill collected from 15 identified sites have been drafted and illustrated. 11 gas station sites with diesel spill were carried out the soil sample collection and 103 diesel spill soil samples were obtained from these sites. Additional 47 polluted soil samples from 9 diesel spill contaminated sites were also provided by private sector and executors of oil tank survey project. Among them, four polluted sites were actually repeated. Hence, there were 150 samples being obtained from 16 contaminated sites in total. Pretreatment of 106 soil samples from 16 diesel spill contaminated sites were carried out and subsequently acquired by GC/MS analysis and data process. All the PCA-DRs cluster analyses for the classification of the diesel oils from the polluted sites were verified for their applications. We have also provided 20 and 25 diagnostic ratio groups, respectively, for the ones without and with the gasoline interferences. The protocols for source oil identification from the diesel spill sites and their operation procedures were suggested. The source investigation and identification of diesel spills from gasoline stations have been completely established. The relative amount of dibenzothiophene derivatives to C4-naphthalenes in the soil extract can be applied for the age-dating preliminary estimate of diesel spill. This project completes the application case analysis of 16 contaminated sites and summarizes the current reference procedures that can be followed. Three technical seminars for the identification and characterization of the oil spill have been held at EPA. The project leader participated in the International Network of Environmental Forensics (INEF) conference held in Beijing in July 2017, and presented the project performed result. One book chapter was published in English and 4 poster presentations were performed in domestic conferences.

英文關鍵字

GC/MS, Measuring Technologies, Principal Component Analysis, Diagnostic ratio, Chemical fingerprint chromatogram, Biomarker, Diesel, Environmental forensics