EPA-104-U1J1-02-102

104

1,800 千元

2015/01/29

2015/12/31

白秀華

環管處

蔡秋美

國立高雄大學

環境衛生

殺蟲劑、鑑定劑量(濃度)、抗藥性、生物檢定

insecticide, discriminating dose or diagnostic dose, resistant, bioassay

徐爾烈

林太明

07-5919755#

minglin9966@gmail.com

以10種殺蟲劑 (賽滅寧、治滅寧、百滅寧、第滅寧、陶斯松、撲滅松、亞特松、安丹、芬普尼及益達胺) 測試本研究室已建立之昆蟲品系進行感藥性測定及建立抗藥性鑑識劑量(濃度) (discriminating dose or diagnostic dose)，提供環境害蟲綜合防治用藥之參考。由生物檢定結果得知本研究室之試驗室品系德國蟑螂對各殺蟲劑之感受性(LD50)順序; 芬普尼(0.11 μg/male)、撲滅松(0.34 μg/male)、第滅寧(0.49 μg/male)、益達胺(1.06 μg/male)、安丹(1.40 μg/male)、陶斯松(1.77 μg/male)、賽滅寧(1.89 μg/male)、亞特松(2.30 μg/male)、百滅寧(5.61 μg/male)、治滅寧(61.24)。試驗室品系美洲蟑螂對各殺蟲劑之感受性(LD50)順序; 第滅寧(0.017 μg/male)、賽滅寧(0.20 μg/male)、安丹(0.37 μg/male)、益達胺(0.99 μg/male)、百滅寧(1.19 μg/male)、芬普尼(3.20) μg/male)、陶斯松(3.29 μg/male)、撲滅松(3.44 μg/male)、亞特松(15.19 μg/male)、治滅寧(81.11)。試驗室品系普通家蠅對各殺蟲劑之感受性(LD50)順序;治滅寧(0.27μg/male)、第滅寧(0.05 μg/male)、芬普尼(0.81 μg/male)、賽滅寧(1.59 μg/male)、亞特松(2.21 μg/male)、百滅寧(2.50 μg/male)、安丹(7.57 μg/male)、撲滅松(9.24 μg/male)、陶斯松(63.81 μg/male)、益達胺(79.42 μg/male)。試驗室品系大頭金蠅對各殺蟲劑之感受性(LD50)順序; 第滅寧(2.52 μg/male)、賽滅寧(10.56 μg/male)、芬普尼(21.96 μg/male)、亞特松(30.25 μg/male)、撲滅松(39.05 μg/male)、百滅寧(39.21 μg/male)、陶斯松(54.22 μg/male)、安丹(66.40 μg/male)、益達胺(146.03 μg/male)、治滅寧(341.7 μg/male)。試驗室品系果蠅對各殺蟲劑之感受性(LD50)順序; 第滅寧(0.02 μg/male)、撲滅松(0.07 μg/male)、賽滅寧(0.23 μg/male)、芬普尼(0.23 μg/male)、益達胺(0.23 μg/male)、陶斯松(0.3 μg/male)、亞特松(0.82 μg/male)、百滅寧(3.57 μg/male)、治滅寧(4.74 μg/male)、安丹(5.90 μg/male)。試驗室品系臭蟲對各殺蟲劑之感受性(LC50)順序; 撲滅松(0.03 ηg/cm2)、陶斯松(0.04 ηg/cm2)、第滅寧(0.14 ηg/cm2)、亞特松(1.36 ηg/cm2)、賽滅寧(2.64 ηg/cm2)、芬普尼(3.47 ηg/cm2)、安丹 (3.62 ηg/cm2)、益達胺(3.92 ηg/cm2)、治滅寧(21.90 ηg/cm2)、百滅寧(46.90 ηg/cm2)。發現德國蟑螂對第滅寧有中抗藥性，其餘9種殺蟲劑是低抗性，美洲蟑螂對10種殺蟲劑皆為低抗藥性；普通家蠅對陶斯松為低抗藥性，其餘9種有高抗藥性及嚴重抗藥性；大頭金蠅對10種殺蟲劑全皆為低抗藥性；果蠅對賽滅寧、芬普尼及益達胺有高抗藥性及嚴重抗藥性；臭蟲對治滅寧及益達胺有高抗藥性。由殺蟲劑感藥性測定分別建立德國蟑螂、美洲蟑螂、普通家蠅、大頭金蠅、果蠅及臭蟲抗藥性鑑識劑量(濃度)，可提供環境害蟲綜合防治時，殺蟲劑劑量(濃度)使用及感藥性鑑識之依據。綜合以上，建立了台灣常見環境害蟲對市售常用10種殺蟲劑之感藥性及抗藥性鑑識劑量(濃度)等之科學依據，可供綜合害蟲防治體系有效殺蟲劑之選用。

殺蟲劑、鑑定劑量(濃度)、抗藥性、生物檢定

104EPA環境害蟲綜合防治計畫.pdf

5MB

2016/2/1 上午 12:00:00

Cypermethrin, tetramethrin, permethrin, deltamethrin, chlorpyrifos, fenitrothion, pirimiphos-methyl, propoxur, fipronil and imidacloprid were tested to the susceptible insect colonies in our laboratory for the determination of the insecticides resistance discriminating dose or diagnostic dose. The results would help the pest control operators to apply more effective control agents. Laboratory of biological assays showed that the order of susceptibilities to various insecticides of laboratory cohort of Bllatela germanica (LD50); fipronil (0.11 μ g/male), fenitrothion (0.34 μ g/male), deltamethrin (0.49 μ g/male), imidacloprid (1.06 μ g/male), propoxur (1.40 μ g/male), chloropyrifos (1.77 μ g/male), cypermethrin (1.89 μ g/male), pirimiphos-methyl (2.30 μ g/male), permethrin (5.61 μ g/male), tetramethrin (61.24). Laboratory cohort of Periplaneta americana showed that the order of susceptibilities to various insecticides (LD50); deltamethrin (0.017 μ g/male), cypermethrin (0.20 μ g/male), propoxur (0.37 μ g/male), imidacloprid (0.99 μ g/male), permethrin (1.19 μ g/male), fipronil (3.20) μ g/male), chloropyrifos (3.29 μg/male), fenitrothion (3.44 μ g/male) , pirimiphos-methyl (15.19 μ g/male), tetramethrin (81.11μ g/male). Laboratory cohort of Musca domestica showed that the order of susceptibilities to various insecticides (LD50); tetramethrin (0.27 μ g/male), deltamethrin (0.05 μ g/male) , fipronil (0.81μg/male) , cypermethrin (1.59μg/male), pirimiphos-methyl (2.21μg/male), permethrin (2.50μg/male) , propoxur (7.57 μg/male), fenitrothion (9.24μg/male) , chloropyrifos (63.81μg/male) ,imidacloprid (79.42μg/ male). Laboratory cohort of Chrysomya megacephala showed that the order of susceptibilities to various insecticides (LD50); deltamethrin (2.52 μ g/male),cypermethrin (10.56 μg/male), fipronil (21.96 μ g/male), pirimiphos-methyl (30.25 μ g/male),permethrin (39.21 μ g/male), chloropyrifos (54.22 μ g/male), propoxur (66.40 μ g/male), imidacloprid (146.03 μ g/male), tetramethrin ( 341.7 μg/male). Llaboratory cohort of Drosophila melanogaster showed that the order of susceptibilities to various insecticides (LD50); deltamethrin (0.02 μ g/male), fenitrothion (0.07 μ g/male), cypermethrin (0.23 μ g/male),fipronil (0.23 μ g/male), imidachloprid (0.23 μ g/male), chloropyrifos (0.3 μ g/male), pirimiphos-methyl (0.82 μ g/male) , permethrin (3.57 μ g/male), tetramethrin (4.74 μ g/male), propoxur (5.90 μ g/male). Laboratory cohort of Cimex lectularius showed that the order of susceptibilities to various insecticides (LC50); fenitrothion (0.03 ηg/cm2), chlorpyifos (0.04 ηg/cm2), deltamethrin (0.14 ηg/cm2), pirmiphos-methyl (1.36 ηg/cm2), cypermethrin (2.64 ηg/cm2), fipronil (3.47 ηg/cm2), propoxur (3.62 ηg/cm2), imidaclorprid (3.92 ηg/cm2), tetramethrin(21.90 ηg/cm2), permethrin (46.90 ηg/cm2). B. germanica, P. americana, C. megacephala, M. domestica, D. melanogaster and C. lectularius were collected from the north, central, south and the east part of Taiwan. The patterns of insecticide susceptibility to cypermethrin, tetramethrin, permethrin, deltamethrin, chlorpyrifos, fenitrothion, pirimiphos-methyl, propoxur, fipronil and imidacloprid by diagnostic dose were determined. B. germanica from collecting study sites were median resistant to deltamethrin but low resistance to other 9 insecticides. P. periplaneta from all study sites were low resistant to all tested insecticides. M. domestica from collecting site were low resistant to chlorpyrifos but high resistant to other 9 insecticides. C. megacephala from all study sites were low resistant to all tested insecticides. D. melanogaster from all study sites were high resistant to cypermethrin, fipronil, and imidachloprid. C. lectularius showed the highest resisitant to imidacloprid. These results provide the scientific basis for integrated pest management systems in effective insecticides used.

insecticide, discriminating dose or diagnostic dose, resistant, bioassay